Cyclo-oxygenase-2 expression in primary cancers of the lung and bladder compared to normal adjacent tissue

Christopher J. Sweeney, Mark S. Marshall, Darlene S. Barnard, Douglas K. Heilman, Steven D. Billings, Liang Cheng, Steven J. Marshall, Michele Yip-Schneider

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Multiple observations in the laboratory and the clinical setting have linked expression of the enzyme cyclo-oxygenase-2 (COX-2) to carcinogenesis. The frequency and amount of COX-2 and Bcl-2 expression in primary lung and bladder cancer sites were determined by immunoblot analysis of cell lysates prepared from frozen human tumor tissue and matched normal adjacent tissue. COX-2 protein was expressed statistically more frequently and at a higher level in primary adenocarcinomas and squamous cell carcinomas of the lung as well as transitional cell carcinomas of the bladder than in normal adjacent tissue. No correlation was observed between COX-2 and Bcl-2 expression in either the lung or bladder cancer specimens. Immunohistochemistry was also employed to localize COX-2 expression. In addition to expression in the malignant tissues, COX-2 was occasionally localized to the normal bronchial and transitional cell epithelia of the normal adjacent tissue. Detection of COX-2 in histologically normal appearing adjacent tissue suggests that COX-2 may be involved in early cellular changes leading to the development of lung and bladder cancer.

Original languageEnglish
Pages (from-to)238-244
Number of pages7
JournalCancer Detection and Prevention
Volume26
Issue number3
DOIs
StatePublished - 2002

Fingerprint

Prostaglandin-Endoperoxide Synthases
Urinary Bladder Neoplasms
Lung Neoplasms
Transitional Cell Carcinoma
Squamous Cell Carcinoma
Carcinogenesis
Urinary Bladder
Adenocarcinoma
Epithelium
Immunohistochemistry
Lung
Enzymes
Neoplasms

Keywords

  • Bcl-2
  • Carcinogenesis
  • COX-2

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Cyclo-oxygenase-2 expression in primary cancers of the lung and bladder compared to normal adjacent tissue. / Sweeney, Christopher J.; Marshall, Mark S.; Barnard, Darlene S.; Heilman, Douglas K.; Billings, Steven D.; Cheng, Liang; Marshall, Steven J.; Yip-Schneider, Michele.

In: Cancer Detection and Prevention, Vol. 26, No. 3, 2002, p. 238-244.

Research output: Contribution to journalArticle

Sweeney, Christopher J. ; Marshall, Mark S. ; Barnard, Darlene S. ; Heilman, Douglas K. ; Billings, Steven D. ; Cheng, Liang ; Marshall, Steven J. ; Yip-Schneider, Michele. / Cyclo-oxygenase-2 expression in primary cancers of the lung and bladder compared to normal adjacent tissue. In: Cancer Detection and Prevention. 2002 ; Vol. 26, No. 3. pp. 238-244.
@article{a73eafd298c04746810bd57c4a22c24f,
title = "Cyclo-oxygenase-2 expression in primary cancers of the lung and bladder compared to normal adjacent tissue",
abstract = "Multiple observations in the laboratory and the clinical setting have linked expression of the enzyme cyclo-oxygenase-2 (COX-2) to carcinogenesis. The frequency and amount of COX-2 and Bcl-2 expression in primary lung and bladder cancer sites were determined by immunoblot analysis of cell lysates prepared from frozen human tumor tissue and matched normal adjacent tissue. COX-2 protein was expressed statistically more frequently and at a higher level in primary adenocarcinomas and squamous cell carcinomas of the lung as well as transitional cell carcinomas of the bladder than in normal adjacent tissue. No correlation was observed between COX-2 and Bcl-2 expression in either the lung or bladder cancer specimens. Immunohistochemistry was also employed to localize COX-2 expression. In addition to expression in the malignant tissues, COX-2 was occasionally localized to the normal bronchial and transitional cell epithelia of the normal adjacent tissue. Detection of COX-2 in histologically normal appearing adjacent tissue suggests that COX-2 may be involved in early cellular changes leading to the development of lung and bladder cancer.",
keywords = "Bcl-2, Carcinogenesis, COX-2",
author = "Sweeney, {Christopher J.} and Marshall, {Mark S.} and Barnard, {Darlene S.} and Heilman, {Douglas K.} and Billings, {Steven D.} and Liang Cheng and Marshall, {Steven J.} and Michele Yip-Schneider",
year = "2002",
doi = "10.1016/S0361-090X(02)00064-8",
language = "English",
volume = "26",
pages = "238--244",
journal = "Cancer Epidemiology",
issn = "1877-7821",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - Cyclo-oxygenase-2 expression in primary cancers of the lung and bladder compared to normal adjacent tissue

AU - Sweeney, Christopher J.

AU - Marshall, Mark S.

AU - Barnard, Darlene S.

AU - Heilman, Douglas K.

AU - Billings, Steven D.

AU - Cheng, Liang

AU - Marshall, Steven J.

AU - Yip-Schneider, Michele

PY - 2002

Y1 - 2002

N2 - Multiple observations in the laboratory and the clinical setting have linked expression of the enzyme cyclo-oxygenase-2 (COX-2) to carcinogenesis. The frequency and amount of COX-2 and Bcl-2 expression in primary lung and bladder cancer sites were determined by immunoblot analysis of cell lysates prepared from frozen human tumor tissue and matched normal adjacent tissue. COX-2 protein was expressed statistically more frequently and at a higher level in primary adenocarcinomas and squamous cell carcinomas of the lung as well as transitional cell carcinomas of the bladder than in normal adjacent tissue. No correlation was observed between COX-2 and Bcl-2 expression in either the lung or bladder cancer specimens. Immunohistochemistry was also employed to localize COX-2 expression. In addition to expression in the malignant tissues, COX-2 was occasionally localized to the normal bronchial and transitional cell epithelia of the normal adjacent tissue. Detection of COX-2 in histologically normal appearing adjacent tissue suggests that COX-2 may be involved in early cellular changes leading to the development of lung and bladder cancer.

AB - Multiple observations in the laboratory and the clinical setting have linked expression of the enzyme cyclo-oxygenase-2 (COX-2) to carcinogenesis. The frequency and amount of COX-2 and Bcl-2 expression in primary lung and bladder cancer sites were determined by immunoblot analysis of cell lysates prepared from frozen human tumor tissue and matched normal adjacent tissue. COX-2 protein was expressed statistically more frequently and at a higher level in primary adenocarcinomas and squamous cell carcinomas of the lung as well as transitional cell carcinomas of the bladder than in normal adjacent tissue. No correlation was observed between COX-2 and Bcl-2 expression in either the lung or bladder cancer specimens. Immunohistochemistry was also employed to localize COX-2 expression. In addition to expression in the malignant tissues, COX-2 was occasionally localized to the normal bronchial and transitional cell epithelia of the normal adjacent tissue. Detection of COX-2 in histologically normal appearing adjacent tissue suggests that COX-2 may be involved in early cellular changes leading to the development of lung and bladder cancer.

KW - Bcl-2

KW - Carcinogenesis

KW - COX-2

UR - http://www.scopus.com/inward/record.url?scp=0036915052&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036915052&partnerID=8YFLogxK

U2 - 10.1016/S0361-090X(02)00064-8

DO - 10.1016/S0361-090X(02)00064-8

M3 - Article

C2 - 12269772

AN - SCOPUS:0036915052

VL - 26

SP - 238

EP - 244

JO - Cancer Epidemiology

JF - Cancer Epidemiology

SN - 1877-7821

IS - 3

ER -