Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p

D. L. Satinover, Gail Vance, D. L. Van Dyke, S. Schwartz

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Over 40 cases of neocentric marker chromosomes, without detectable α-satellite DNA, have been reported. Although these have originated from many different chromosomes, a few of these chromosomes have been involved in multiple cases of marker formation. In this study, two different markers originating from the short arm of chromosome 9 were analyzed, identifying a common neocentrometric region. A bacterial artificial chromosome (BAC) contig extending over more than 900 kb has been assembled across this neocentromeric region. Fluorescent in situ hybridization and immunofluorescence assays (CENP-C and CENP-E) have localized the neocentromere to a 500 kb region. Preliminary analysis of DNA sequences in this neocentromere revealed a highly AT-rich region, which also has an increase in the level of retroviral elements compared with the average levels in the genome.

Original languageEnglish (US)
Pages (from-to)275-283
Number of pages9
JournalChromosoma
Volume110
Issue number4
StatePublished - 2001

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Bacterial Artificial Chromosomes
Cytogenetic Analysis
Chromosomes
AT Rich Sequence
Satellite DNA
Chromosomes, Human, Pair 9
Fluorescence In Situ Hybridization
DNA Sequence Analysis
Genetic Markers
Fluorescent Antibody Technique
Genome
centromere protein C

ASJC Scopus subject areas

  • Genetics

Cite this

Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p. / Satinover, D. L.; Vance, Gail; Van Dyke, D. L.; Schwartz, S.

In: Chromosoma, Vol. 110, No. 4, 2001, p. 275-283.

Research output: Contribution to journalArticle

Satinover, DL, Vance, G, Van Dyke, DL & Schwartz, S 2001, 'Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p', Chromosoma, vol. 110, no. 4, pp. 275-283.
Satinover, D. L. ; Vance, Gail ; Van Dyke, D. L. ; Schwartz, S. / Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p. In: Chromosoma. 2001 ; Vol. 110, No. 4. pp. 275-283.
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