Cytogenetic analysis and construction of a BAC contig across a common neocentromeric region from 9p

D. L. Satinover, G. H. Vance, D. L. Van Dyke, S. Schwartz

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26 Scopus citations

Abstract

Over 40 cases of neocentric marker chromosomes, without detectable α-satellite DNA, have been reported. Although these have originated from many different chromosomes, a few of these chromosomes have been involved in multiple cases of marker formation. In this study, two different markers originating from the short arm of chromosome 9 were analyzed, identifying a common neocentrometric region. A bacterial artificial chromosome (BAC) contig extending over more than 900 kb has been assembled across this neocentromeric region. Fluorescent in situ hybridization and immunofluorescence assays (CENP-C and CENP-E) have localized the neocentromere to a 500 kb region. Preliminary analysis of DNA sequences in this neocentromere revealed a highly AT-rich region, which also has an increase in the level of retroviral elements compared with the average levels in the genome.

Original languageEnglish (US)
Pages (from-to)275-283
Number of pages9
JournalChromosoma
Volume110
Issue number4
DOIs
StatePublished - Jan 1 2001

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ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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