Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE

Jill Reiter, Holli M. Drendel, Sujata Chakraborty, Megan M. Schellinger, Men Jean Lee, Gil Mor

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Immortalization of primary cells with telomerase is thought to maintain normal phenotypic properties and avoid chromosomal abnormalities and other cancer-associated changes that occur following simian virus 40 tumor antigen (SV40 Tag) induced immortalization. However, we report that the human telomerase reverse transcriptase (hTERT)-immortalized SWAN-71 trophoblast cell line has a near pentaploid 103∼119,XXXX[cp20] karyotype. Additionally, DNA typing analysis indicated that SWAN-71 cells have acquired microsatellite instability. In comparison, the post-crisis SV40-transformed trophoblast cell line 3A-subE was hypertriploid 69∼81,XX[cp20]. Both cell lines contained multiple specific clonal rearrangements. These findings emphasize the need to monitor for genetic instability in hTERT-immortalized cells.

Original languageEnglish (US)
Pages (from-to)17-20
Number of pages4
JournalPlacenta
Volume52
DOIs
StatePublished - Apr 1 2017

Keywords

  • Cellular immortalization
  • Karyotype
  • Polyploidy
  • Short tandem repeat profile
  • Telomerase
  • Trophoblast cell lines

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

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  • Cite this

    Reiter, J., Drendel, H. M., Chakraborty, S., Schellinger, M. M., Lee, M. J., & Mor, G. (2017). Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE. Placenta, 52, 17-20. https://doi.org/10.1016/j.placenta.2017.02.010