Cytokine mediated expansion of human umbilical cord blood CD34+ cells

Comparison of the use of partially purified and pure CD34+ target cells

Jamie Case, A. Rice, M. Vowels

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To determine the optimal cell population for cytokine mediated expansion, we compared the use of Magnetic Cell Sorting (MACS) system enriched CD34+ human umbilical cord blood (HUCB) cells with that of MACS enriched, flow purified CD34+ HUCB cells. Both MACS enriched CD34+ cells and MACS enriched, flow purified CD34+ cells (mean starting purity of CD34+ SC 51.27 ± 7.6% and 96.36 ± 1.34% respectively n = 6) were incubated for seven days with Interleukin-1 (IL-1)+IL-3+Stem Cell Factor (SCF) and showed a fold increase in the number of nucleated cells (10.02 ± 2.6 and 18.23 ± 4.73 respectively) and a reduction in the percentage of CD34+ cells (5.55 ± 1.23% and 12.21 ± 3.29% respectively). An increase in the absolute numbers of CD34+ cells (4.8 x 104 ± 2.3 x 104) was observed with MACS enriched CD34+ cells as compared to no change (1.3 x 105 ± 8.8 x 104) with MACS enriched, flow purified CD34+ cells. An increase in IL-3+GM-CSF+SCF responsive colony forming unit (CFU) (1.7 x 104 ± 9.4 x 103 and 1.6 x 105 ± 7.7 x 104 respectively) was also observed as compared with input values (1.5 x 104 ± 1 x 104 and 2.3 x 104 ± 8.9 x 103 respectively). We conclude that MACS enriched, flow sorted CD34+ HUCB cells have greater cytokine mediated expansion potential as measured by progenitor expansion, than MACS enriched CD34+ HUCB cells.

Original languageEnglish (US)
Pages (from-to)193-197
Number of pages5
JournalHematology and Cell Therapy
Volume39
Issue number4
DOIs
StatePublished - Aug 1997
Externally publishedYes

Fingerprint

Fetal Blood
Blood Cells
Cytokines
Stem Cell Factor
Interleukin-3
Cell Count
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-1
Stem Cells

Keywords

  • Cord blood CD34 progenitor cells
  • Cytokine mediated expansion

ASJC Scopus subject areas

  • Hematology

Cite this

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title = "Cytokine mediated expansion of human umbilical cord blood CD34+ cells: Comparison of the use of partially purified and pure CD34+ target cells",
abstract = "To determine the optimal cell population for cytokine mediated expansion, we compared the use of Magnetic Cell Sorting (MACS) system enriched CD34+ human umbilical cord blood (HUCB) cells with that of MACS enriched, flow purified CD34+ HUCB cells. Both MACS enriched CD34+ cells and MACS enriched, flow purified CD34+ cells (mean starting purity of CD34+ SC 51.27 ± 7.6{\%} and 96.36 ± 1.34{\%} respectively n = 6) were incubated for seven days with Interleukin-1 (IL-1)+IL-3+Stem Cell Factor (SCF) and showed a fold increase in the number of nucleated cells (10.02 ± 2.6 and 18.23 ± 4.73 respectively) and a reduction in the percentage of CD34+ cells (5.55 ± 1.23{\%} and 12.21 ± 3.29{\%} respectively). An increase in the absolute numbers of CD34+ cells (4.8 x 104 ± 2.3 x 104) was observed with MACS enriched CD34+ cells as compared to no change (1.3 x 105 ± 8.8 x 104) with MACS enriched, flow purified CD34+ cells. An increase in IL-3+GM-CSF+SCF responsive colony forming unit (CFU) (1.7 x 104 ± 9.4 x 103 and 1.6 x 105 ± 7.7 x 104 respectively) was also observed as compared with input values (1.5 x 104 ± 1 x 104 and 2.3 x 104 ± 8.9 x 103 respectively). We conclude that MACS enriched, flow sorted CD34+ HUCB cells have greater cytokine mediated expansion potential as measured by progenitor expansion, than MACS enriched CD34+ HUCB cells.",
keywords = "Cord blood CD34 progenitor cells, Cytokine mediated expansion",
author = "Jamie Case and A. Rice and M. Vowels",
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TY - JOUR

T1 - Cytokine mediated expansion of human umbilical cord blood CD34+ cells

T2 - Comparison of the use of partially purified and pure CD34+ target cells

AU - Case, Jamie

AU - Rice, A.

AU - Vowels, M.

PY - 1997/8

Y1 - 1997/8

N2 - To determine the optimal cell population for cytokine mediated expansion, we compared the use of Magnetic Cell Sorting (MACS) system enriched CD34+ human umbilical cord blood (HUCB) cells with that of MACS enriched, flow purified CD34+ HUCB cells. Both MACS enriched CD34+ cells and MACS enriched, flow purified CD34+ cells (mean starting purity of CD34+ SC 51.27 ± 7.6% and 96.36 ± 1.34% respectively n = 6) were incubated for seven days with Interleukin-1 (IL-1)+IL-3+Stem Cell Factor (SCF) and showed a fold increase in the number of nucleated cells (10.02 ± 2.6 and 18.23 ± 4.73 respectively) and a reduction in the percentage of CD34+ cells (5.55 ± 1.23% and 12.21 ± 3.29% respectively). An increase in the absolute numbers of CD34+ cells (4.8 x 104 ± 2.3 x 104) was observed with MACS enriched CD34+ cells as compared to no change (1.3 x 105 ± 8.8 x 104) with MACS enriched, flow purified CD34+ cells. An increase in IL-3+GM-CSF+SCF responsive colony forming unit (CFU) (1.7 x 104 ± 9.4 x 103 and 1.6 x 105 ± 7.7 x 104 respectively) was also observed as compared with input values (1.5 x 104 ± 1 x 104 and 2.3 x 104 ± 8.9 x 103 respectively). We conclude that MACS enriched, flow sorted CD34+ HUCB cells have greater cytokine mediated expansion potential as measured by progenitor expansion, than MACS enriched CD34+ HUCB cells.

AB - To determine the optimal cell population for cytokine mediated expansion, we compared the use of Magnetic Cell Sorting (MACS) system enriched CD34+ human umbilical cord blood (HUCB) cells with that of MACS enriched, flow purified CD34+ HUCB cells. Both MACS enriched CD34+ cells and MACS enriched, flow purified CD34+ cells (mean starting purity of CD34+ SC 51.27 ± 7.6% and 96.36 ± 1.34% respectively n = 6) were incubated for seven days with Interleukin-1 (IL-1)+IL-3+Stem Cell Factor (SCF) and showed a fold increase in the number of nucleated cells (10.02 ± 2.6 and 18.23 ± 4.73 respectively) and a reduction in the percentage of CD34+ cells (5.55 ± 1.23% and 12.21 ± 3.29% respectively). An increase in the absolute numbers of CD34+ cells (4.8 x 104 ± 2.3 x 104) was observed with MACS enriched CD34+ cells as compared to no change (1.3 x 105 ± 8.8 x 104) with MACS enriched, flow purified CD34+ cells. An increase in IL-3+GM-CSF+SCF responsive colony forming unit (CFU) (1.7 x 104 ± 9.4 x 103 and 1.6 x 105 ± 7.7 x 104 respectively) was also observed as compared with input values (1.5 x 104 ± 1 x 104 and 2.3 x 104 ± 8.9 x 103 respectively). We conclude that MACS enriched, flow sorted CD34+ HUCB cells have greater cytokine mediated expansion potential as measured by progenitor expansion, than MACS enriched CD34+ HUCB cells.

KW - Cord blood CD34 progenitor cells

KW - Cytokine mediated expansion

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JO - Annals of Hematology

JF - Annals of Hematology

SN - 0939-5555

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ER -