The natural killer activity and phenotypic properties of six different subpopulations of normal human peripheral blood lymphocytes obtained by four-color immunofluorescence cell sorting were examined. Phycoerythrin-conjugated CD16 and CD56 were used simultaneously to identify (CD16+CD56+) NK cells. The cells most effective in mediating NK cytolysis against K562 target cells were CD3-(16+56+)57-8+. Although most of the K562 killing was found in the CD3-(16+56+) groups of cells, a substantial degree of NK activity was detected in the CD3+(16+56+) subpopulations of some individuals. The level of expression of CD57 and CD8 was significantly higher on CD3+(16+56+) than on CD3-(16+56+) cells.
- large granular lymphocytes
- multiparameter flow cytometric cell sorting
- NK subpopulations
ASJC Scopus subject areas
- Cell Biology
- Pathology and Forensic Medicine