Cytolytic activity of human natural killer cell subpopulations isolated by four-color immunofluorescence flow cytometric cell sorting

Edward Srour, T. Leemhuis, L. Jenski, R. Redmond, J. Jansen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The natural killer activity and phenotypic properties of six different subpopulations of normal human peripheral blood lymphocytes obtained by four-color immunofluorescence cell sorting were examined. Phycoerythrin-conjugated CD16 and CD56 were used simultaneously to identify (CD16+CD56+) NK cells. The cells most effective in mediating NK cytolysis against K562 target cells were CD3-(16+56+)57-8+. Although most of the K562 killing was found in the CD3-(16+56+) groups of cells, a substantial degree of NK activity was detected in the CD3+(16+56+) subpopulations of some individuals. The level of expression of CD57 and CD8 was significantly higher on CD3+(16+56+) than on CD3-(16+56+) cells.

Original languageEnglish
Pages (from-to)442-446
Number of pages5
JournalCytometry
Volume11
Issue number3
StatePublished - 1990

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Human Activities
Natural Killer Cells
Fluorescent Antibody Technique
Color
Phycoerythrin
K562 Cells
Lymphocytes

Keywords

  • large granular lymphocytes
  • multiparameter flow cytometric cell sorting
  • NK subpopulations

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Cytolytic activity of human natural killer cell subpopulations isolated by four-color immunofluorescence flow cytometric cell sorting. / Srour, Edward; Leemhuis, T.; Jenski, L.; Redmond, R.; Jansen, J.

In: Cytometry, Vol. 11, No. 3, 1990, p. 442-446.

Research output: Contribution to journalArticle

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