Cytolytic activity of human natural killer cell subpopulations isolated by four‐color immunofluorescence flow cytometric cell sorting

Edward F. Srour, Tom Leemhuis, Laura Jenski, Robert Redmond, Jan Jansen

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The natural killer activity and phenotypic properties of six different subpopulations of normal human peripheral blood lymphocytes obtained by four-color immunofluorescence cell sorting were examined. Phycoerythrin-conjugated CD16 and CD56 were used simultaneously to identify (CD16+CD56+) NK cells. The cells most effective in mediating NK cytolysis against K562 target cells were CD3-(16+56+)57-8+. Although most of the K562 killing was found in the CD3-(16+56+) groups of cells, a substantial degree of NK activity was detected in the CD3+(16+56+) subpopulations of some individuals. The level of expression of CD57 and CD8 was significantly higher on CD3+(16+56+) than on CD3-(16+56+) cells.

Original languageEnglish (US)
Pages (from-to)442-446
Number of pages5
JournalCytometry
Volume11
Issue number3
DOIs
StatePublished - 1990

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Keywords

  • large granular lymphocytes
  • multiparameter flow cytometric cell sorting
  • NK subpopulations

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

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