Daily phototherapy with red light to regulate candida albicans biofilm growth

Beatriz H.D. Panariello, Bruna A. Garcia, Simone Duarte

Research output: Contribution to journalArticle

Abstract

Here, we present a protocol to assess the outcomes of per diem red light treatment on the growth of Candida albicans biofilm. To increase the planktonic growth of C. albicans SN425, the inoculums grew on Yeast Nitrogen Base media. For biofilm formation, RPMI 1640 media, which have high concentrations of amino acids, were applied to help biofilm growth. Biofilms of 48 h were treated twice a day for a period of 1 min with a non-coherent light device (red light; wavelength = 635 nm; energy density = 87.6 J·cm -2 ). As a positive control (PC), 0.12% chlorhexidine (CHX) was applied, and as a negative control (NC), 0.89% NaCl was applied to the biofilms. Colony forming units (CFU), dry-weight, soluble and insoluble exopolysaccharides were quantified after treatments. Briefly, the protocol presented here is simple, reproducible and provides answers regarding viability, dry-weight and extracellular polysaccharide amounts after red light treatment.

Original languageEnglish (US)
Article numbere59326
JournalJournal of Visualized Experiments
Volume2019
Issue number146
DOIs
StatePublished - Apr 2019

Keywords

  • Biofilm
  • Candida albicans
  • Extracellular matrix
  • Immunology and Infection
  • Issue 146
  • Phototherapy
  • Polyssacharides
  • Red light

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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