Specific immunohistochemical techniques are often required to better understand bone pathology as well as normal growth and repair. Various tissue-processing methods can enhance or erode antigen preservation. Here, we examine the decalcification of slide-mounted sections of undecalcified bone embedded in methylmethacrylate by using ethylenediaminetetracetate, formic acid, or hydrochloric acid, in addition to routine immunohistochemical localization procedures. Ethylenediaminetetracetate decalcification allowed for improved antigen staining in select sections, while not demineralizing, and therefore possibly compromising, the whole bone specimen.
- Bone sialoprotein
ASJC Scopus subject areas
- Medical Laboratory Technology