Decorin-binding protein A (DbpA) of Borrelia burgdorferi is not protective when immunized mice are challenged via tick infestation and correlates with the lack of DbpA expression by B. burgdorferi in ticks

Kayla E. Hagman, X. Yang, Stephen K. Wikel, George B. Schoeler, Melissa J. Caimano, Justin D. Radolf, Michael V. Norgard

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Previous studies showed that decorin-binding protein A (DbpA) of Borrelia burgdorferi was a protective immunogen in the murine model of Lyme borreliosis when mice were challenged (needle inoculated) intradermally with in vitro-cultivated spirochetes. In the present study, DbpA-immunized C3H/HeJ mice were not protected from infection when infested with Ixodes scapularis nymphs harboring virulent B. burgdorferi 297. This lack of protection correlated with the failure to detect DbpA on B. burgdorferi in ticks, suggesting that DbpA is not available as a target for bactericidal antibodies in serum when B. burgdorferi-infected ticks take their blood meal from an immunized host. The failure of DbpA immunization to protect tick-challenged mice contradicts the results of earlier needle inoculation vaccination experiments and suggests that DbpA may not be suitable as a Lyme disease vaccine.

Original languageEnglish (US)
Pages (from-to)4759-4764
Number of pages6
JournalInfection and Immunity
Volume68
Issue number8
DOIs
StatePublished - 2000
Externally publishedYes

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Tick Infestations
Decorin
Borrelia burgdorferi
Staphylococcal Protein A
Ticks
Carrier Proteins
Needles
Lyme Disease Vaccines
Nymph
Ixodes
Spirochaetales
Lyme Disease
Inbred C3H Mouse
Meals
Immunization
Vaccination
Antibodies
Infection
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Decorin-binding protein A (DbpA) of Borrelia burgdorferi is not protective when immunized mice are challenged via tick infestation and correlates with the lack of DbpA expression by B. burgdorferi in ticks. / Hagman, Kayla E.; Yang, X.; Wikel, Stephen K.; Schoeler, George B.; Caimano, Melissa J.; Radolf, Justin D.; Norgard, Michael V.

In: Infection and Immunity, Vol. 68, No. 8, 2000, p. 4759-4764.

Research output: Contribution to journalArticle

Hagman, Kayla E. ; Yang, X. ; Wikel, Stephen K. ; Schoeler, George B. ; Caimano, Melissa J. ; Radolf, Justin D. ; Norgard, Michael V. / Decorin-binding protein A (DbpA) of Borrelia burgdorferi is not protective when immunized mice are challenged via tick infestation and correlates with the lack of DbpA expression by B. burgdorferi in ticks. In: Infection and Immunity. 2000 ; Vol. 68, No. 8. pp. 4759-4764.
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abstract = "Previous studies showed that decorin-binding protein A (DbpA) of Borrelia burgdorferi was a protective immunogen in the murine model of Lyme borreliosis when mice were challenged (needle inoculated) intradermally with in vitro-cultivated spirochetes. In the present study, DbpA-immunized C3H/HeJ mice were not protected from infection when infested with Ixodes scapularis nymphs harboring virulent B. burgdorferi 297. This lack of protection correlated with the failure to detect DbpA on B. burgdorferi in ticks, suggesting that DbpA is not available as a target for bactericidal antibodies in serum when B. burgdorferi-infected ticks take their blood meal from an immunized host. The failure of DbpA immunization to protect tick-challenged mice contradicts the results of earlier needle inoculation vaccination experiments and suggests that DbpA may not be suitable as a Lyme disease vaccine.",
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AU - Norgard, Michael V.

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