Abstract
Human immunodeficiency virus type 1 (HIV-1) infection is associated with B cell activation and exhaustion, and hypergammaglobulinemia. How these changes influence B cell responses to coinfections such as malaria is poorly understood. To address this, we compared B cell phenotypes and Abs specific for the Plasmodium falciparum vaccine candidate apical membrane Ag-1 (AMA1) in HIV-infected and uninfected adults living in Kenya. Surprisingly, HIV-1 infection was not associated with a difference in serum AMA1-specific Ab levels. HIV-infected individuals had a higher proportion of total atypical and total activated memory B cells (MBCs). Using an AMA1 tetramer to detect AMA1-specific B cells, HIV-infected individuals were also shown to have a higher proportion of AMA1-specific atypical MBCs. However, this proportional increase resulted in large part from a loss in the number of naive and resting MBCs rather than an increase in the number of atypical and activated cells. The loss of resting MBCs and naive B cells was mirrored in a population of cells specific for an Ag to which these individuals were unlikely to have been chronically exposed. Together, the data show that changes in P. falciparum Ag-specific B cell subsets in HIV-infected individuals mirror those in the overall B cell population, and suggest that the increased proportion of atypical MBC phenotypes found in HIV- 1-infected individuals results from the loss of naive and resting MBCs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4629-4638 |
| Number of pages | 10 |
| Journal | Journal of Immunology |
| Volume | 198 |
| Issue number | 12 |
| DOIs | |
| State | Published - Jun 15 2017 |
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ASJC Scopus subject areas
- Immunology
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Decrease in numbers of naive and resting B cells in HIV-infected Kenyan Adults leads to a proportional increase in total and plasmodium falciparum-specific atypical memory B cells. / Frosch, Anne E.; Odumade, Oludare A.; Taylor, Justin J.; Ireland, Kathleen; Ayodo, George; Ondigo, Bartholomew; Narum, David L.; Vulule, John; John, Chandy.
In: Journal of Immunology, Vol. 198, No. 12, 15.06.2017, p. 4629-4638.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Decrease in numbers of naive and resting B cells in HIV-infected Kenyan Adults leads to a proportional increase in total and plasmodium falciparum-specific atypical memory B cells
AU - Frosch, Anne E.
AU - Odumade, Oludare A.
AU - Taylor, Justin J.
AU - Ireland, Kathleen
AU - Ayodo, George
AU - Ondigo, Bartholomew
AU - Narum, David L.
AU - Vulule, John
AU - John, Chandy
PY - 2017/6/15
Y1 - 2017/6/15
N2 - Human immunodeficiency virus type 1 (HIV-1) infection is associated with B cell activation and exhaustion, and hypergammaglobulinemia. How these changes influence B cell responses to coinfections such as malaria is poorly understood. To address this, we compared B cell phenotypes and Abs specific for the Plasmodium falciparum vaccine candidate apical membrane Ag-1 (AMA1) in HIV-infected and uninfected adults living in Kenya. Surprisingly, HIV-1 infection was not associated with a difference in serum AMA1-specific Ab levels. HIV-infected individuals had a higher proportion of total atypical and total activated memory B cells (MBCs). Using an AMA1 tetramer to detect AMA1-specific B cells, HIV-infected individuals were also shown to have a higher proportion of AMA1-specific atypical MBCs. However, this proportional increase resulted in large part from a loss in the number of naive and resting MBCs rather than an increase in the number of atypical and activated cells. The loss of resting MBCs and naive B cells was mirrored in a population of cells specific for an Ag to which these individuals were unlikely to have been chronically exposed. Together, the data show that changes in P. falciparum Ag-specific B cell subsets in HIV-infected individuals mirror those in the overall B cell population, and suggest that the increased proportion of atypical MBC phenotypes found in HIV- 1-infected individuals results from the loss of naive and resting MBCs.
AB - Human immunodeficiency virus type 1 (HIV-1) infection is associated with B cell activation and exhaustion, and hypergammaglobulinemia. How these changes influence B cell responses to coinfections such as malaria is poorly understood. To address this, we compared B cell phenotypes and Abs specific for the Plasmodium falciparum vaccine candidate apical membrane Ag-1 (AMA1) in HIV-infected and uninfected adults living in Kenya. Surprisingly, HIV-1 infection was not associated with a difference in serum AMA1-specific Ab levels. HIV-infected individuals had a higher proportion of total atypical and total activated memory B cells (MBCs). Using an AMA1 tetramer to detect AMA1-specific B cells, HIV-infected individuals were also shown to have a higher proportion of AMA1-specific atypical MBCs. However, this proportional increase resulted in large part from a loss in the number of naive and resting MBCs rather than an increase in the number of atypical and activated cells. The loss of resting MBCs and naive B cells was mirrored in a population of cells specific for an Ag to which these individuals were unlikely to have been chronically exposed. Together, the data show that changes in P. falciparum Ag-specific B cell subsets in HIV-infected individuals mirror those in the overall B cell population, and suggest that the increased proportion of atypical MBC phenotypes found in HIV- 1-infected individuals results from the loss of naive and resting MBCs.
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U2 - 10.4049/jimmunol.1600773
DO - 10.4049/jimmunol.1600773
M3 - Article
C2 - 28526680
AN - SCOPUS:85020480162
VL - 198
SP - 4629
EP - 4638
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 12
ER -