Decrease in numbers of naive and resting B cells in HIV-infected Kenyan Adults leads to a proportional increase in total and plasmodium falciparum-specific atypical memory B cells

Anne E. Frosch, Oludare A. Odumade, Justin J. Taylor, Kathleen Ireland, George Ayodo, Bartholomew Ondigo, David L. Narum, John Vulule, Chandy John

Research output: Contribution to journalArticle

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Abstract

Human immunodeficiency virus type 1 (HIV-1) infection is associated with B cell activation and exhaustion, and hypergammaglobulinemia. How these changes influence B cell responses to coinfections such as malaria is poorly understood. To address this, we compared B cell phenotypes and Abs specific for the Plasmodium falciparum vaccine candidate apical membrane Ag-1 (AMA1) in HIV-infected and uninfected adults living in Kenya. Surprisingly, HIV-1 infection was not associated with a difference in serum AMA1-specific Ab levels. HIV-infected individuals had a higher proportion of total atypical and total activated memory B cells (MBCs). Using an AMA1 tetramer to detect AMA1-specific B cells, HIV-infected individuals were also shown to have a higher proportion of AMA1-specific atypical MBCs. However, this proportional increase resulted in large part from a loss in the number of naive and resting MBCs rather than an increase in the number of atypical and activated cells. The loss of resting MBCs and naive B cells was mirrored in a population of cells specific for an Ag to which these individuals were unlikely to have been chronically exposed. Together, the data show that changes in P. falciparum Ag-specific B cell subsets in HIV-infected individuals mirror those in the overall B cell population, and suggest that the increased proportion of atypical MBC phenotypes found in HIV- 1-infected individuals results from the loss of naive and resting MBCs.

Original languageEnglish (US)
Pages (from-to)4629-4638
Number of pages10
JournalJournal of Immunology
Volume198
Issue number12
DOIs
StatePublished - Jun 15 2017

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Plasmodium falciparum
B-Lymphocytes
HIV
HIV-1
Membranes
Virus Diseases
B-Lymphocyte Subsets
Hypergammaglobulinemia
Phenotype
Kenya
Memory Disorders
Coinfection
Population
Malaria
Vaccines

ASJC Scopus subject areas

  • Immunology

Cite this

Decrease in numbers of naive and resting B cells in HIV-infected Kenyan Adults leads to a proportional increase in total and plasmodium falciparum-specific atypical memory B cells. / Frosch, Anne E.; Odumade, Oludare A.; Taylor, Justin J.; Ireland, Kathleen; Ayodo, George; Ondigo, Bartholomew; Narum, David L.; Vulule, John; John, Chandy.

In: Journal of Immunology, Vol. 198, No. 12, 15.06.2017, p. 4629-4638.

Research output: Contribution to journalArticle

Frosch, Anne E. ; Odumade, Oludare A. ; Taylor, Justin J. ; Ireland, Kathleen ; Ayodo, George ; Ondigo, Bartholomew ; Narum, David L. ; Vulule, John ; John, Chandy. / Decrease in numbers of naive and resting B cells in HIV-infected Kenyan Adults leads to a proportional increase in total and plasmodium falciparum-specific atypical memory B cells. In: Journal of Immunology. 2017 ; Vol. 198, No. 12. pp. 4629-4638.
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