Decreased aquaporin expression leads to increased resistance to apoptosis in hepatocellular carcinoma

Elizabeth M. Jablonski, M. Adrian Mattocks, Eugene Sokolov, Leonidas G. Koniaris, Francis M. Hughes, Nelson Fausto, Robert H. Pierce, Iain H. McKillop

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

Cells undergoing apoptosis are characterized by decreased cell size due to changes in intracellular ion concentration and rapid, aquaporin (AQP)-dependent water movement out of the cell, events required for the activation of pro-apoptotic enzymes. The current study demonstrates AQP 8 and 9 expression is significantly decreased in hepatocellular carcinoma (HCC) versus normal liver. Isolation of hepatic tumor cells (H4IIE) and hepatocytes confirmed a lack of water movement across the H4IIE cell membrane via AQPs and identified an inherent resistance of H4IIE cells to apoptotic stimuli. In contrast, hepatocytes rapidly responded to osmotic challenge through AQP-dependent water movement and underwent cell death following apoptotic stimulation.

Original languageEnglish (US)
Pages (from-to)36-46
Number of pages11
JournalCancer Letters
Volume250
Issue number1
DOIs
StatePublished - May 18 2007
Externally publishedYes

Keywords

  • Apoptosis
  • Apoptotic volume decrease
  • Aquaporin
  • Hepatocellular carcinoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Jablonski, E. M., Adrian Mattocks, M., Sokolov, E., Koniaris, L. G., Hughes, F. M., Fausto, N., Pierce, R. H., & McKillop, I. H. (2007). Decreased aquaporin expression leads to increased resistance to apoptosis in hepatocellular carcinoma. Cancer Letters, 250(1), 36-46. https://doi.org/10.1016/j.canlet.2006.09.013