Decreased cerebrospinal-fluid/serum leptin ratio in obesity

A possible mechanism for leptin resistance

José F. Caro, Jerzy W. Kolaczynski, Mark R. Nyce, Joanna P. Ohannesian, Irina Opentanova, Warren H. Goldman, Richard B. Lynn, Pei Li Zhang, Madhur K. Sinha, Robert Considine

Research output: Contribution to journalArticle

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Abstract

Background. A receptor for leptin has been cloned from the choroid plexus, the site of cerebrospinal-fluid (CSF) production and the location of the blood/cerebrospinal-fluid barrier. Thus, this receptor might serve as a transporter for leptin. We have studied leptin concentrations in serum and (CSF). Methods and findings. We demonstrated by radioimmunoassay and western blot the presence of leptin in human CSF. We then measured leptin in CSF and serum in 31 individuals with a wide range of bodyweight. Mean serum leptin was 318% higher in 8 obese (40.2 [SE 8.6] ng/mL) than in 23 lean individuals (9.6 [1.5] ng/mL, p <0.0005). However, the CSF leptin concentration in obese individuals (0.337 [0.04] ng/mL) was only 30% higher than in lean people (0.259 [0.26] ng/mL, p <0.1). Consequently, the leptin CSF/serum ratio in lean individuals (0.047 [0.010]) was 4.3-fold higher than that in obese individuals (0.011 [0.002], p <0.05). The relation between CSF leptin and serum leptin was best described by a logarithmic function (r = 0.52, p <0.01). Interpretation. Our data suggest that leptin enters the brain by a saturable transport system. The capacity of leptin transport is lower in obese individuals, and may provide a mechanism for leptin resistance.

Original languageEnglish (US)
Pages (from-to)159-161
Number of pages3
JournalThe Lancet
Volume348
Issue number9021
DOIs
StatePublished - Jul 20 1996
Externally publishedYes

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Leptin
Cerebrospinal Fluid
Obesity
Serum
Leptin Receptors
Choroid Plexus
Radioimmunoassay
Western Blotting

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Caro, J. F., Kolaczynski, J. W., Nyce, M. R., Ohannesian, J. P., Opentanova, I., Goldman, W. H., ... Considine, R. (1996). Decreased cerebrospinal-fluid/serum leptin ratio in obesity: A possible mechanism for leptin resistance. The Lancet, 348(9021), 159-161. https://doi.org/10.1016/S0140-6736(96)03173-X

Decreased cerebrospinal-fluid/serum leptin ratio in obesity : A possible mechanism for leptin resistance. / Caro, José F.; Kolaczynski, Jerzy W.; Nyce, Mark R.; Ohannesian, Joanna P.; Opentanova, Irina; Goldman, Warren H.; Lynn, Richard B.; Zhang, Pei Li; Sinha, Madhur K.; Considine, Robert.

In: The Lancet, Vol. 348, No. 9021, 20.07.1996, p. 159-161.

Research output: Contribution to journalArticle

Caro, JF, Kolaczynski, JW, Nyce, MR, Ohannesian, JP, Opentanova, I, Goldman, WH, Lynn, RB, Zhang, PL, Sinha, MK & Considine, R 1996, 'Decreased cerebrospinal-fluid/serum leptin ratio in obesity: A possible mechanism for leptin resistance', The Lancet, vol. 348, no. 9021, pp. 159-161. https://doi.org/10.1016/S0140-6736(96)03173-X
Caro JF, Kolaczynski JW, Nyce MR, Ohannesian JP, Opentanova I, Goldman WH et al. Decreased cerebrospinal-fluid/serum leptin ratio in obesity: A possible mechanism for leptin resistance. The Lancet. 1996 Jul 20;348(9021):159-161. https://doi.org/10.1016/S0140-6736(96)03173-X
Caro, José F. ; Kolaczynski, Jerzy W. ; Nyce, Mark R. ; Ohannesian, Joanna P. ; Opentanova, Irina ; Goldman, Warren H. ; Lynn, Richard B. ; Zhang, Pei Li ; Sinha, Madhur K. ; Considine, Robert. / Decreased cerebrospinal-fluid/serum leptin ratio in obesity : A possible mechanism for leptin resistance. In: The Lancet. 1996 ; Vol. 348, No. 9021. pp. 159-161.
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AU - Goldman, Warren H.

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