Decreased inflammatory response in Toll-like receptor 2 knockout mice is associated with exacerbated Pneumocystis pneumonia

Shao Hung Wang, Chen Zhang, Mark E. Lasbury, Chung Ping Liao, Pamela J. Durant, Dennis Tschang, Chao-Hung Lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Pneumocystis pneumonia (PcP) is marked by substantial inflammatory damage to the lung. We have found that Toll-like receptor 2 (TLR2) mediates macrophage inflammatory responses to Pneumocystis and hypothesized that TLR2 deficiency would lead to less severe inflammation and milder lung injury during PcP. Histopathology examination showed that TLR2-/- mice with PcP indeed exhibited milder pulmonary inflammation. TLR2-/- mouse lungs contained less TNF-α and displayed lower levels of NF-κB activation during PcP. However, TLR2-/- mice with PcP displayed increased severity in symptoms and organism burden. The increased organism burden is likely due to defects in protective mechanisms in TLR2-/- mice. mRNA levels of the inducible nitric oxide synthase and NADPH oxidase p47phox, as well as nitric oxide levels in the lungs, were decreased in TLR2-/- PcP mice. Taken together, this study shows that TLR2-mediated inflammatory responses contribute to a certain degree to the clearance of Pneumocystis organism in mice.

Original languageEnglish
Pages (from-to)334-341
Number of pages8
JournalMicrobes and Infection
Volume10
Issue number4
DOIs
StatePublished - Apr 2008

Fingerprint

Toll-Like Receptor 2
Pneumocystis Pneumonia
Knockout Mice
Pneumocystis
Lung
NADPH Oxidase
Lung Injury
Nitric Oxide Synthase Type II
Pneumonia
Nitric Oxide
Macrophages
Inflammation
Messenger RNA
Mouse Tlr2 protein

Keywords

  • Inflammation
  • Pneumocystis
  • Toll-like receptor 2

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Decreased inflammatory response in Toll-like receptor 2 knockout mice is associated with exacerbated Pneumocystis pneumonia. / Wang, Shao Hung; Zhang, Chen; Lasbury, Mark E.; Liao, Chung Ping; Durant, Pamela J.; Tschang, Dennis; Lee, Chao-Hung.

In: Microbes and Infection, Vol. 10, No. 4, 04.2008, p. 334-341.

Research output: Contribution to journalArticle

Wang, Shao Hung ; Zhang, Chen ; Lasbury, Mark E. ; Liao, Chung Ping ; Durant, Pamela J. ; Tschang, Dennis ; Lee, Chao-Hung. / Decreased inflammatory response in Toll-like receptor 2 knockout mice is associated with exacerbated Pneumocystis pneumonia. In: Microbes and Infection. 2008 ; Vol. 10, No. 4. pp. 334-341.
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