Decreased p38 MAPK activity in end-stage failing human myocardium: p38 MAPK α is the predominant isoform expressed in human heart

Leslie E. Lemke, Laura J. Bloem, Rebecca Fouts, Michail Esterman, George Sandusky, Chris J. Vlahos

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Short duration exposure to cellular stresses have been shown to activate p38 mitogen-activated protein kinase (MAPK) in cultured rat ventricular cardiomyocytes and isolated perfused hearts; however, effects of chronic stress on p38 MAPK are not well understood. This study determined whether alterations in the p38 MAPK pathway occurred prior to end-stage human heart failure. The p38 MAPK α isoform was detectable in human cardiac tissue. However, carefully controlled analysis of protein and message in this study demonstrated an absence of the p38 MAPK β-isoform. Low levels of message for the non-SB203580 sensitive p38 MAPK γ and δ isoforms were also detected in both normal and failing human myocardium. Ischemic and idiopathic end-stage failing human hearts were compared to non-failing hearts for both p38 α MAPK protein level and total p38 MAPK activity. Western blotting techniques demonstrated no significant changes in total p38 α MAPK content. However, approximately 75% decreases in active/phosphorylated p38 MAPK (P

Original languageEnglish (US)
Pages (from-to)1527-1540
Number of pages14
JournalJournal of Molecular and Cellular Cardiology
Volume33
Issue number8
DOIs
StatePublished - 2001
Externally publishedYes

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p38 Mitogen-Activated Protein Kinases
Myocardium
Protein Isoforms
Cardiac Myocytes
Proteins
Heart Failure
Western Blotting

Keywords

  • Congestive heart failure
  • Hypertrophy
  • Mitogen activated protein kinase
  • SB203580
  • Signal transduction

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Decreased p38 MAPK activity in end-stage failing human myocardium : p38 MAPK α is the predominant isoform expressed in human heart. / Lemke, Leslie E.; Bloem, Laura J.; Fouts, Rebecca; Esterman, Michail; Sandusky, George; Vlahos, Chris J.

In: Journal of Molecular and Cellular Cardiology, Vol. 33, No. 8, 2001, p. 1527-1540.

Research output: Contribution to journalArticle

Lemke, Leslie E. ; Bloem, Laura J. ; Fouts, Rebecca ; Esterman, Michail ; Sandusky, George ; Vlahos, Chris J. / Decreased p38 MAPK activity in end-stage failing human myocardium : p38 MAPK α is the predominant isoform expressed in human heart. In: Journal of Molecular and Cellular Cardiology. 2001 ; Vol. 33, No. 8. pp. 1527-1540.
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