Decreased platelet reactivity identified by whole blood flow cytometry in Fanconi anaemia patients

Susanne Holzhauer, Ana Gabriela Sitaru, Wolfram Ebell, Detlev Schindler, Helmut Hanenberg, Johannes Wirbelauer, Ulrich Walter, Ralf Grossmann

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Fanconi anaemia (FA) is a rare inherited chromosome instability disorder characterized by congenital anomalies and a high risk for bone marrow failure and cancer. Bleeding is a frequent complication in FA, leading to substantial morbidity and mortality. Thrombocytopenia is a major factor leading to this complication, but the bleeding tendency of FA patients often exceeds what one might expect based on their platelet counts. We therefore investigated if alterations of platelet function contribute to the bleeding tendency of FA patients. We assessed platelet function in I I FA patients and 23 controls with whole blood flow cytometry. We analyzed the expression of platelet membrane glycoprotein receptors, reactivity of platelets to physiologic agonists and the proportion of young platelets. In FA patients platelet reactivity was decreased: Expression of P-selectin and binding of PAC-I after stimulation with thrombin receptor activating peptide (TRAP) and adenosine diphosphate (ADP) were 15-70% lower than in controls. We found no or only minor differences of platelet glycoprotein receptor expression between groups. While the proportion of reticulated platelets was not different, the absolute number of reticulated platelets was markedly lower in FA patients. Our data show that FA is associated with reduced platelet reactivity, which may contribute to the high bleeding tendency in FA patients. Whole blood flow cytometry is a suitable method for analysis of platelet function in FA patient

Original languageEnglish (US)
Pages (from-to)1291-1297
Number of pages7
JournalThrombosis and Haemostasis
Volume98
Issue number6
DOIs
StatePublished - Dec 2007
Externally publishedYes

Fingerprint

Fanconi Anemia
Flow Cytometry
Blood Platelets
Hemorrhage
Platelet Membrane Glycoproteins
thrombin receptor peptide SFLLRNP
Platelet Count
Chromosome Disorders
Bone Neoplasms
Chromosomal Instability
P-Selectin
Thrombocytopenia
Adenosine Diphosphate
Bone Marrow
Morbidity

Keywords

  • Bleeding risk
  • Fanconi anaemia
  • Flow cytometry
  • Platelet function
  • Thrombocytopenia

ASJC Scopus subject areas

  • Hematology

Cite this

Holzhauer, S., Sitaru, A. G., Ebell, W., Schindler, D., Hanenberg, H., Wirbelauer, J., ... Grossmann, R. (2007). Decreased platelet reactivity identified by whole blood flow cytometry in Fanconi anaemia patients. Thrombosis and Haemostasis, 98(6), 1291-1297. https://doi.org/10.1160/TH07-06-0426

Decreased platelet reactivity identified by whole blood flow cytometry in Fanconi anaemia patients. / Holzhauer, Susanne; Sitaru, Ana Gabriela; Ebell, Wolfram; Schindler, Detlev; Hanenberg, Helmut; Wirbelauer, Johannes; Walter, Ulrich; Grossmann, Ralf.

In: Thrombosis and Haemostasis, Vol. 98, No. 6, 12.2007, p. 1291-1297.

Research output: Contribution to journalArticle

Holzhauer, S, Sitaru, AG, Ebell, W, Schindler, D, Hanenberg, H, Wirbelauer, J, Walter, U & Grossmann, R 2007, 'Decreased platelet reactivity identified by whole blood flow cytometry in Fanconi anaemia patients', Thrombosis and Haemostasis, vol. 98, no. 6, pp. 1291-1297. https://doi.org/10.1160/TH07-06-0426
Holzhauer S, Sitaru AG, Ebell W, Schindler D, Hanenberg H, Wirbelauer J et al. Decreased platelet reactivity identified by whole blood flow cytometry in Fanconi anaemia patients. Thrombosis and Haemostasis. 2007 Dec;98(6):1291-1297. https://doi.org/10.1160/TH07-06-0426
Holzhauer, Susanne ; Sitaru, Ana Gabriela ; Ebell, Wolfram ; Schindler, Detlev ; Hanenberg, Helmut ; Wirbelauer, Johannes ; Walter, Ulrich ; Grossmann, Ralf. / Decreased platelet reactivity identified by whole blood flow cytometry in Fanconi anaemia patients. In: Thrombosis and Haemostasis. 2007 ; Vol. 98, No. 6. pp. 1291-1297.
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