Deep tissue fluorescent imaging in scattering specimens using confocal microscopy

Sherry G. Clendenon, Pamela A. Young, Michael Ferkowicz, Carrie Phillips, Kenneth W. Dunn

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or multiphoton microscopy. Tissue clearing appears to eliminate the need for multiphoton excitation for deep tissue imaging.

Original languageEnglish (US)
Pages (from-to)614-617
Number of pages4
JournalMicroscopy and Microanalysis
Volume17
Issue number4
DOIs
StatePublished - Aug 1 2011

Keywords

  • clearing
  • confocal
  • multiphoton
  • refractive index matching
  • scattering

ASJC Scopus subject areas

  • Instrumentation

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