Deep tissue fluorescent imaging in scattering specimens using confocal microscopy

Sherry G. Clendenon, Pamela A. Young, Michael Ferkowicz, Carrie Phillips, Kenneth Dunn

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or multiphoton microscopy. Tissue clearing appears to eliminate the need for multiphoton excitation for deep tissue imaging.

Original languageEnglish
Pages (from-to)614-617
Number of pages4
JournalMicroscopy and Microanalysis
Volume17
Issue number4
DOIs
StatePublished - Aug 2011

Fingerprint

clearing
Confocal microscopy
Scattering
Tissue
microscopy
Imaging techniques
scattering
excitation
Image quality
Microscopic examination

Keywords

  • clearing
  • confocal
  • multiphoton
  • refractive index matching
  • scattering

ASJC Scopus subject areas

  • Instrumentation

Cite this

Deep tissue fluorescent imaging in scattering specimens using confocal microscopy. / Clendenon, Sherry G.; Young, Pamela A.; Ferkowicz, Michael; Phillips, Carrie; Dunn, Kenneth.

In: Microscopy and Microanalysis, Vol. 17, No. 4, 08.2011, p. 614-617.

Research output: Contribution to journalArticle

Clendenon, Sherry G. ; Young, Pamela A. ; Ferkowicz, Michael ; Phillips, Carrie ; Dunn, Kenneth. / Deep tissue fluorescent imaging in scattering specimens using confocal microscopy. In: Microscopy and Microanalysis. 2011 ; Vol. 17, No. 4. pp. 614-617.
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