Defect in neutrophil killing and increased susceptibility to infection with nonpathogenic gram-positive bacteria in peptidoglycan recognition protein-S (PGRP-S)-deficient mice

Roman Dziarski, Kenneth A. Platt, Eva Gelius, Håkan Steiner, Dipika Gupta

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Insect peptidoglycan recognition protein-S (PGRP-S), a member of a family of innate immunity pattern recognition molecules conserved from insects to mammals, recognizes bacterial cell wall peptidoglycan and activates 2 antimicrobial defense systems, prophenoloxidase cascade and antimicrobial peptides through Toll receptor. We show that mouse PGRP-S is present in neutrophil tertiary granules and that PGRP-S-deficient (PGRP-S-/-) mice have increased susceptibility to intraperitoneal infection with gram-positive bacteria of low pathogenicity but not with more pathogenic grampositive or gram-negative bacteria. PGRP-S-/- mice have normal inflammatory responses and production of tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6). Neutrophils from PGRP-S-/- mice have normal phagocytic uptake of bacteria but are defective in intracellular killing and digestion of relatively nonpathogenic gram-positive bacteria. Therefore, mammalian PGRP-S functions in intracellular killing of bacteria. Thus, only bacterial recognition by PGRP-S, but not its effector function, is conserved from insects to mammals.

Original languageEnglish
Pages (from-to)689-697
Number of pages9
JournalBlood
Volume102
Issue number2
DOIs
StatePublished - Jul 15 2003

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Protein S
Gram-Positive Bacteria
Bacteria
Neutrophils
Defects
Infection
Insects
Mammals
Peptidoglycan
peptidoglycan recognition protein
Gram-Negative Bacteria
Innate Immunity
Cell Wall
Pattern recognition
Virulence
Digestion
Interleukin-6
Tumor Necrosis Factor-alpha
Cells
Peptides

ASJC Scopus subject areas

  • Hematology

Cite this

Defect in neutrophil killing and increased susceptibility to infection with nonpathogenic gram-positive bacteria in peptidoglycan recognition protein-S (PGRP-S)-deficient mice. / Dziarski, Roman; Platt, Kenneth A.; Gelius, Eva; Steiner, Håkan; Gupta, Dipika.

In: Blood, Vol. 102, No. 2, 15.07.2003, p. 689-697.

Research output: Contribution to journalArticle

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