Definitive hematopoietic commitment within the embryonic vascular endothelial-cadherin+ population

Stuart T. Fraser, Minetaro Ogawa, Ruth T. Yu, Satomi Nishikawa, Mervin Yoder, Shin Ichi Nishikawa

Research output: Contribution to journalArticle

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Abstract

Objective. The aim of this study was to assess the potential of FLK1+ and vascular endothelial (VE)-cadherin+ populations from different stages of embryonic development to generate hematopoietic cells ex vivo and to contribute to the hematopoietic systems of recipient mice. Materials and Methods. FLK1+ of VE-cadherin+ cells were isolated from 7.5- to 9.5-dpc concepti and cultured ex vivo on OP9 stromal cells and hematopoietic development examined. VE-cadherin+CD45- cells from 8.5- and 9.5-dpc concepti were injected intrahepatically into newborn busulfan-treated SCID recipients and engraftment monitored. Results. VE-cadherin+ cells from 7.5- and 8.5-dpc concepti can readily generate hematopoi-etic cells ex vivo compared to FLK1+ VE-cadherin- cells. Similar hematopoietic potential can be found in the VE-cadherin+ cells from the 8.5-dpc yolk sac. When VE-cadherin+CD45- cells were injected into SCID recipients, long-term engraftment, particularly within the lymphoid system, was observed. This potential was observed in VE-cadherin+CD45- cells from 9.5-dpc embryo or yolk sac but from tissues from younger concepti. Conclusions. FLK1+VE-cadherin- cells, possibly representing the lateral plate mesoderm, are not as effective at generating hematopoietic cells compared to similarly staged VE-cadherin+ cells. VE-cadherin+CD45- cells can also contribute to the hematolymphoid system of intrahepatically injected newborn SCID recipients, suggesting that cells bearing an endothelial phenotype are capable of generating long-term hematopoietic precursors.

Original languageEnglish (US)
Pages (from-to)1070-1078
Number of pages9
JournalExperimental Hematology
Volume30
Issue number9
DOIs
StatePublished - Sep 2002
Externally publishedYes

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Endothelial Cells
Population
Yolk Sac
cadherin 5
Hematopoietic System
Busulfan
Mesoderm
Stromal Cells
Embryonic Development
Embryonic Structures
Phenotype

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Definitive hematopoietic commitment within the embryonic vascular endothelial-cadherin+ population. / Fraser, Stuart T.; Ogawa, Minetaro; Yu, Ruth T.; Nishikawa, Satomi; Yoder, Mervin; Nishikawa, Shin Ichi.

In: Experimental Hematology, Vol. 30, No. 9, 09.2002, p. 1070-1078.

Research output: Contribution to journalArticle

Fraser, Stuart T. ; Ogawa, Minetaro ; Yu, Ruth T. ; Nishikawa, Satomi ; Yoder, Mervin ; Nishikawa, Shin Ichi. / Definitive hematopoietic commitment within the embryonic vascular endothelial-cadherin+ population. In: Experimental Hematology. 2002 ; Vol. 30, No. 9. pp. 1070-1078.
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