Degree of antigen adsorption in the vaccine or interstitial fluid and its effect on the antibody response in rabbits

Mei Fang Chang, Yi Shi, Steven L. Nail, Harm HogenEsch, Stephen B. Adams, Joe L. White, Stanley L. Hem

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

The effect of the degree of adsorption of lysozyme by aluminium hydroxide adjuvant on the immune response in rabbits was studied. The surface charge of the adjuvant was modified by pretreatment with phosphate anion to produce five vaccines having degrees of adsorption ranging from 3 to 90%. The degree of adsorption of vaccines exhibiting 3, 35 or 85% adsorption changed to 40% within 1 h after each vaccine was mixed with sheep interstitial fluid to simulate subcutaneous administration. The mean anti-lysozyme antibody titers produced by the vaccines were the same and were four times greater than that produced by a lysozyme solution. Thus, the degree of adsorption of lysozyme in sheep interstitial fluid rather than the degree of adsorption in the vaccine correlated with the immune response.

Original languageEnglish (US)
Pages (from-to)2884-2889
Number of pages6
JournalVaccine
Volume19
Issue number20-22
DOIs
StatePublished - Apr 6 2001
Externally publishedYes

Keywords

  • Aluminium hydroxide adjuvant
  • Degree of adsorption in interstitial fluid
  • Degree of adsorption in vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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