Degree of antigen adsorption in the vaccine or interstitial fluid and its effect on the antibody response in rabbits

Mei Fang Chang, Yi Shi, Steven L. Nail, Harm HogenEsch, Stephen B. Adams, Joe L. White, Stanley L. Hem

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The effect of the degree of adsorption of lysozyme by aluminium hydroxide adjuvant on the immune response in rabbits was studied. The surface charge of the adjuvant was modified by pretreatment with phosphate anion to produce five vaccines having degrees of adsorption ranging from 3 to 90%. The degree of adsorption of vaccines exhibiting 3, 35 or 85% adsorption changed to 40% within 1 h after each vaccine was mixed with sheep interstitial fluid to simulate subcutaneous administration. The mean anti-lysozyme antibody titers produced by the vaccines were the same and were four times greater than that produced by a lysozyme solution. Thus, the degree of adsorption of lysozyme in sheep interstitial fluid rather than the degree of adsorption in the vaccine correlated with the immune response.

Original languageEnglish (US)
Pages (from-to)2884-2889
Number of pages6
JournalVaccine
Volume19
Issue number20-22
DOIs
StatePublished - Apr 6 2001
Externally publishedYes

Fingerprint

extracellular fluids
Extracellular Fluid
Adsorption
Antibody Formation
adsorption
Vaccines
rabbits
Muramidase
vaccines
Rabbits
antigens
lysozyme
Antigens
antibodies
adjuvants
Sheep
immune response
Aluminum Hydroxide
sheep
aluminum hydroxide

Keywords

  • Aluminium hydroxide adjuvant
  • Degree of adsorption in interstitial fluid
  • Degree of adsorption in vaccine

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Degree of antigen adsorption in the vaccine or interstitial fluid and its effect on the antibody response in rabbits. / Chang, Mei Fang; Shi, Yi; Nail, Steven L.; HogenEsch, Harm; Adams, Stephen B.; White, Joe L.; Hem, Stanley L.

In: Vaccine, Vol. 19, No. 20-22, 06.04.2001, p. 2884-2889.

Research output: Contribution to journalArticle

Chang, Mei Fang ; Shi, Yi ; Nail, Steven L. ; HogenEsch, Harm ; Adams, Stephen B. ; White, Joe L. ; Hem, Stanley L. / Degree of antigen adsorption in the vaccine or interstitial fluid and its effect on the antibody response in rabbits. In: Vaccine. 2001 ; Vol. 19, No. 20-22. pp. 2884-2889.
@article{a30b3bc2fa7747d896789d379d51e32a,
title = "Degree of antigen adsorption in the vaccine or interstitial fluid and its effect on the antibody response in rabbits",
abstract = "The effect of the degree of adsorption of lysozyme by aluminium hydroxide adjuvant on the immune response in rabbits was studied. The surface charge of the adjuvant was modified by pretreatment with phosphate anion to produce five vaccines having degrees of adsorption ranging from 3 to 90{\%}. The degree of adsorption of vaccines exhibiting 3, 35 or 85{\%} adsorption changed to 40{\%} within 1 h after each vaccine was mixed with sheep interstitial fluid to simulate subcutaneous administration. The mean anti-lysozyme antibody titers produced by the vaccines were the same and were four times greater than that produced by a lysozyme solution. Thus, the degree of adsorption of lysozyme in sheep interstitial fluid rather than the degree of adsorption in the vaccine correlated with the immune response.",
keywords = "Aluminium hydroxide adjuvant, Degree of adsorption in interstitial fluid, Degree of adsorption in vaccine",
author = "Chang, {Mei Fang} and Yi Shi and Nail, {Steven L.} and Harm HogenEsch and Adams, {Stephen B.} and White, {Joe L.} and Hem, {Stanley L.}",
year = "2001",
month = "4",
day = "6",
doi = "10.1016/S0264-410X(00)00559-4",
language = "English (US)",
volume = "19",
pages = "2884--2889",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "20-22",

}

TY - JOUR

T1 - Degree of antigen adsorption in the vaccine or interstitial fluid and its effect on the antibody response in rabbits

AU - Chang, Mei Fang

AU - Shi, Yi

AU - Nail, Steven L.

AU - HogenEsch, Harm

AU - Adams, Stephen B.

AU - White, Joe L.

AU - Hem, Stanley L.

PY - 2001/4/6

Y1 - 2001/4/6

N2 - The effect of the degree of adsorption of lysozyme by aluminium hydroxide adjuvant on the immune response in rabbits was studied. The surface charge of the adjuvant was modified by pretreatment with phosphate anion to produce five vaccines having degrees of adsorption ranging from 3 to 90%. The degree of adsorption of vaccines exhibiting 3, 35 or 85% adsorption changed to 40% within 1 h after each vaccine was mixed with sheep interstitial fluid to simulate subcutaneous administration. The mean anti-lysozyme antibody titers produced by the vaccines were the same and were four times greater than that produced by a lysozyme solution. Thus, the degree of adsorption of lysozyme in sheep interstitial fluid rather than the degree of adsorption in the vaccine correlated with the immune response.

AB - The effect of the degree of adsorption of lysozyme by aluminium hydroxide adjuvant on the immune response in rabbits was studied. The surface charge of the adjuvant was modified by pretreatment with phosphate anion to produce five vaccines having degrees of adsorption ranging from 3 to 90%. The degree of adsorption of vaccines exhibiting 3, 35 or 85% adsorption changed to 40% within 1 h after each vaccine was mixed with sheep interstitial fluid to simulate subcutaneous administration. The mean anti-lysozyme antibody titers produced by the vaccines were the same and were four times greater than that produced by a lysozyme solution. Thus, the degree of adsorption of lysozyme in sheep interstitial fluid rather than the degree of adsorption in the vaccine correlated with the immune response.

KW - Aluminium hydroxide adjuvant

KW - Degree of adsorption in interstitial fluid

KW - Degree of adsorption in vaccine

UR - http://www.scopus.com/inward/record.url?scp=0035815521&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035815521&partnerID=8YFLogxK

U2 - 10.1016/S0264-410X(00)00559-4

DO - 10.1016/S0264-410X(00)00559-4

M3 - Article

VL - 19

SP - 2884

EP - 2889

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 20-22

ER -