Delayed correlation of mRNA and protein expression in rapamycin-treated cells and a role for Ggc1 in cellular sensitivity to rapamycin

Marjorie L. Fournier, Ariel Paulson, Norman Pavelka, Amber Mosley, Karin Gaudenz, William D. Bradford, Earl Glynn, Hua Li, Mihaela E. Sardiu, Brian Fleharty, Christopher Seidel, Laurence Florens, Michael P. Washburn

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

To identify new molecular targets of rapamycin, an anticancer and immunosuppressive drug, we analyzed temporal changes in yeast over 6 h in response to rapamycin at the transcriptome and proteome levels and integrated the expression patterns with functional profiling. We show that the integration of transcriptomics, proteomics, and functional data sets provides novel insights into the molecular mechanisms of rapamycin action. We first observed a temporal delay in the correlation of mRNA and protein expression where mRNA expression at 1 and 2 h correlated best with protein expression changes after 6 h of rapamycin treatment. This was especially the case for the inhibition of ribosome biogenesis and induction of heat shock and autophagy essential to promote the cellular sensitivity to rapamycin. However, increased levels of vacuolar protease could enhance resistance to rapamycin. Of the 85 proteins identified as statistically significantly changing in abundance, most of the proteins that decreased in abundance were correlated with a decrease in mRNA expression. However, of the 56 proteins increasing in abundance, 26 were not correlated with an increase in mRNA expression. These protein changes were correlated with unchanged or down-regulated mRNA expression. These proteins, involved in mitochondrial genome maintenance, endocytosis, or drug export, represent new candidates effecting rapamycin action whose expression might be post-transcriptionally or post-translationally regulated. We identified GGC1, a mitochondrial GTP/GDP carrier, as a new component of the rapamycin/target of rapamycin (TOR) signaling pathway. We determined that the protein product of GGC1 was stabilized in the presence of rapamycin, and the deletion of the GGC1 enhanced growth fitness in the presence of rapamycin. A dynamic mRNA expression analysis of △ggc1 and wildtype cells treated with rapamycin revealed a key role for Ggc1p in the regulation of ribosome biogenesis and cell cycle progression under TOR control.

Original languageEnglish (US)
Pages (from-to)271-284
Number of pages14
JournalMolecular & cellular proteomics : MCP
Volume9
Issue number2
DOIs
StatePublished - Feb 2010
Externally publishedYes

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Sirolimus
Cells
Messenger RNA
Proteins
Ribosomes
Mitochondrial Genome
Autophagy
Proteome
Immunosuppressive Agents
Endocytosis
Guanosine Triphosphate
Transcriptome
Pharmaceutical Preparations
Proteomics
Yeast
Shock
Cell Cycle
Peptide Hydrolases
Hot Temperature
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Cite this

Delayed correlation of mRNA and protein expression in rapamycin-treated cells and a role for Ggc1 in cellular sensitivity to rapamycin. / Fournier, Marjorie L.; Paulson, Ariel; Pavelka, Norman; Mosley, Amber; Gaudenz, Karin; Bradford, William D.; Glynn, Earl; Li, Hua; Sardiu, Mihaela E.; Fleharty, Brian; Seidel, Christopher; Florens, Laurence; Washburn, Michael P.

In: Molecular & cellular proteomics : MCP, Vol. 9, No. 2, 02.2010, p. 271-284.

Research output: Contribution to journalArticle

Fournier, ML, Paulson, A, Pavelka, N, Mosley, A, Gaudenz, K, Bradford, WD, Glynn, E, Li, H, Sardiu, ME, Fleharty, B, Seidel, C, Florens, L & Washburn, MP 2010, 'Delayed correlation of mRNA and protein expression in rapamycin-treated cells and a role for Ggc1 in cellular sensitivity to rapamycin', Molecular & cellular proteomics : MCP, vol. 9, no. 2, pp. 271-284. https://doi.org/10.1074/mcp.M900415-MCP200
Fournier, Marjorie L. ; Paulson, Ariel ; Pavelka, Norman ; Mosley, Amber ; Gaudenz, Karin ; Bradford, William D. ; Glynn, Earl ; Li, Hua ; Sardiu, Mihaela E. ; Fleharty, Brian ; Seidel, Christopher ; Florens, Laurence ; Washburn, Michael P. / Delayed correlation of mRNA and protein expression in rapamycin-treated cells and a role for Ggc1 in cellular sensitivity to rapamycin. In: Molecular & cellular proteomics : MCP. 2010 ; Vol. 9, No. 2. pp. 271-284.
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AU - Bradford, William D.

AU - Glynn, Earl

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AU - Sardiu, Mihaela E.

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AU - Seidel, Christopher

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