Deletion of bid impedes cell proliferation and hepatic carcinogenesis

Li Bai, Hong Min Ni, Xiaoyun Chen, Daniell Difrancesca, Xiao Ming Yin

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Mechanisms that control the proliferation capability of the initiated cells during hepatocarcinogenesis are still largely unclear. We investigated the role of a pro-death Bcl-2 family protein, Bid, in liver tumor development using a neonatal diethylnitrosamine model. Diethylnitrosamine was administrated to 15-day-old wild-type and bid-null mice. The development of microfoci at the early stage and of gross tumors at the later stage was compared between the two groups of mice. Both microfoci and gross tumor development were significantly retarded in the bid-null mice, despite reduced cell death as measured by TUNEL staining. Further studies indicated that there were significantly less proliferating cells in diethylnitrosamine-treated bid-null livers. The regulation of cell proliferation by Bid was confirmed in two other systems not involving carcinogenesis. Hepatocyte proliferation following partial hepatectomy and T lymphocyte proliferation following anti-CD3 stimulation were both retarded in bid-null mice. Thus, these studies revealed a previously undisclosed function of Bid in regulating cell proliferation, which can be important to tumor development. Furthermore, the role of Bid in promoting hepatocarcinogenesis is in contrast to its reported role in suppressing myeloid leukemia and thus suggests an organ- and/or etiology-specific role of the Bcl-2 family proteins in regulating oncogenesis.

Original languageEnglish (US)
Pages (from-to)1523-1532
Number of pages10
JournalAmerican Journal of Pathology
Volume166
Issue number5
DOIs
StatePublished - May 2005

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Diethylnitrosamine
Carcinogenesis
Cell Proliferation
Liver
Neoplasms
BH3 Interacting Domain Death Agonist Protein
Myeloid Leukemia
In Situ Nick-End Labeling
Hepatectomy
Hepatocytes
Cell Death
Staining and Labeling
T-Lymphocytes
Proteins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Deletion of bid impedes cell proliferation and hepatic carcinogenesis. / Bai, Li; Ni, Hong Min; Chen, Xiaoyun; Difrancesca, Daniell; Yin, Xiao Ming.

In: American Journal of Pathology, Vol. 166, No. 5, 05.2005, p. 1523-1532.

Research output: Contribution to journalArticle

Bai, Li ; Ni, Hong Min ; Chen, Xiaoyun ; Difrancesca, Daniell ; Yin, Xiao Ming. / Deletion of bid impedes cell proliferation and hepatic carcinogenesis. In: American Journal of Pathology. 2005 ; Vol. 166, No. 5. pp. 1523-1532.
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