Delphinidin induces necrosis in hepatocellular carcinoma cells in the presence of 3-methyladenine, an autophagy inhibitor

Rentian Feng, Shiow Y. Wang, Ying Hong Shi, Jia Fan, Xiao Ming Yin

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

The present study was performed to determine whether anthocyanins could trigger different modes of cell death in different cancers. It was found that whereas cyanidin-3-rutinoside and delphinidin could induce apoptosis in leukemia cells, they caused growth retardation in hepatocellular carcinoma cells (HCC), which was accompanied with a significant cellular vacuolization. The latter was likely caused by macroautophagy and was completely suppressed by 3-methyladenine, an inhibitor of class III phosphoinositide 3-kinase that is important for autophagy activation, and by bafilomycin A1, which blocks lysosomal degradation. Delphinidin induced significant lipidation of LC3, an indication of macroautophagy, which was also suppressed by 3-methyladenine. Macroautophagy was required for the survival of delphinidin-treated HCC cells as inhibition with 3-methyladenine led to massive necrosis without caspase activation. Thus, anthocyanins could induce different modes of cell death for different cancers. Furthermore, anthocyanins could be used in combination with a macroautophagy inhibitor for treating cancers such as HCC.

Original languageEnglish (US)
Pages (from-to)3957-3964
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number7
DOIs
StatePublished - Apr 14 2010

Keywords

  • Autophagy
  • Bilberry
  • Delphinidin
  • Hepatocellular carcinoma
  • Necrosis

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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