Demonstration that cyclic adenosine 3',5' monophosphate mediates the lipolytic action of parathyroid hormone

P. Thajchayapong, Sherry Queener, R. McClintock, D. O. Allen, N. H. Bell

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Studies were carried out with rat epididymal fat pads first to compare the effects of the synthetic N terminal 1-34 peptide of bovine parathyroid hormone and of the native hormone to determine whether this portion of the molecule is responsible for the lipolytic action of the hormone and second to determine whether this biologic action of parathyroid hormone is mediated by cyclic adenosine 3',5' monophosphate. The N terminal polypeptide was as effective as the native hormone in stimulating lipolysis in the concentration range between 10 -8M and 10 -6M. Parathyroid hormone stimulated lipolysis by isolated fat cells. The concentration of cyclic adenosine 3',5' monophosphate in the fat pads was significantly decreased by the hormone (10 -6M). Lipolytic stimulation by parathyroid hormone (10 -6M) was diminished by insulin (100 μU/ml) and prostaglandin E 1 (1 μg/ml), both of which are known inhibitors of lipolysis. The findings indicate that the amino terminal 1-34 peptide portion of parathyroid hormone is responsible for the lipolytic action and that this effect is mediated through cyclic adenosine 3',5' monophosphate.

Original languageEnglish
Pages (from-to)190-195
Number of pages6
JournalHormone and Metabolic Research
Volume8
Issue number3
StatePublished - 1976

Fingerprint

Parathyroid Hormone
Cyclic AMP
Lipolysis
Demonstrations
Hormones
Fats
Adipose Tissue
Peptides
Prostaglandins E
Adipocytes
Rats
Insulin
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Demonstration that cyclic adenosine 3',5' monophosphate mediates the lipolytic action of parathyroid hormone. / Thajchayapong, P.; Queener, Sherry; McClintock, R.; Allen, D. O.; Bell, N. H.

In: Hormone and Metabolic Research, Vol. 8, No. 3, 1976, p. 190-195.

Research output: Contribution to journalArticle

Thajchayapong, P. ; Queener, Sherry ; McClintock, R. ; Allen, D. O. ; Bell, N. H. / Demonstration that cyclic adenosine 3',5' monophosphate mediates the lipolytic action of parathyroid hormone. In: Hormone and Metabolic Research. 1976 ; Vol. 8, No. 3. pp. 190-195.
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