Dendritic cells armed with anti-CD3 mAbs reduce pulmonary metastases, prolong survival, and engender antitumor effector cells demonstrable by adoptive transfer

Mary Maluccio, Jyosthna Rao, Vijay Sharma, Milagros Lagman, Manikkam Suthanthiran

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Dendritic cells (DCs) pulsed with tumor cells or peptides are effective antitumor agents in a number of tumor models. In light of our earlier demonstration that T-cell signaling via the CD3 proteins induces cytolytic activity and constrains tumor progression, we equipped DCs pulsed with tumor cells with anti-CD3 mAbs and tested their antitumor efficacy in a murine renal cell cancer pulmonary metastasis model. Methods: We investigated the antitumor efficacy of DCs pulsed with whole irradiated tumor cells (DC/R) or DCs pulsed with irradiated tumor cells and armed with anti-CD3 mAbs (DC/R/anti-CD3 mAbs). Experimental end points included the number of pulmonary metastases and survival of tumor-inoculated mice. Results: Our studies demonstrate that arming tumor-pulsed DCs with anti-CD3 mAbs results in a superior outcome compared to that from tumor-pulsed DCs alone in terms of reduction in the number of pulmonary metastases and survival times. Furthermore, adoptive transfer experiments revealed that the splenocytes from DC/R/anti-CD3 mAbs-treated mice are superior to splenocytes from DC/R-treated mice in reducing renal cancer pulmonary metastases in severe combined immunodeficient (SCID) beige mice. Conclusion: Our data suggest that the therapeutic efficacy of DCs pulsed with tumor cells can be augmented by arming them with anti-CD3 mAbs. DC-based treatment regimens that currently are being pursued in clinical trials might be improved by equipping such cells with anti-CD3 mAbs.

Original languageEnglish (US)
Pages (from-to)771-776
Number of pages6
JournalAnnals of Surgical Oncology
Volume7
Issue number10
StatePublished - 2000
Externally publishedYes

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Adoptive Transfer
Dendritic Cells
Neoplasm Metastasis
Lung
Neoplasms
SCID Mice
Kidney Neoplasms
Renal Cell Carcinoma
Antineoplastic Agents
Clinical Trials

Keywords

  • Anti-CD3 mAbs
  • Dendritic cells
  • Tumor immunotherapy

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

Dendritic cells armed with anti-CD3 mAbs reduce pulmonary metastases, prolong survival, and engender antitumor effector cells demonstrable by adoptive transfer. / Maluccio, Mary; Rao, Jyosthna; Sharma, Vijay; Lagman, Milagros; Suthanthiran, Manikkam.

In: Annals of Surgical Oncology, Vol. 7, No. 10, 2000, p. 771-776.

Research output: Contribution to journalArticle

Maluccio, Mary ; Rao, Jyosthna ; Sharma, Vijay ; Lagman, Milagros ; Suthanthiran, Manikkam. / Dendritic cells armed with anti-CD3 mAbs reduce pulmonary metastases, prolong survival, and engender antitumor effector cells demonstrable by adoptive transfer. In: Annals of Surgical Oncology. 2000 ; Vol. 7, No. 10. pp. 771-776.
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