Dendritic cells produce inflammatory cytokines in response to bacterial products from Staphylococcus aureus-infected atopic dermatitis lesions

Timothy Voorhees, Jihoon Chang, Yongxue Yao, Mark Kaplan, Cheong Hee Chang, Jeffrey Travers

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Atopic Dermatitis (AD) patients often acquire secondary skin infections resulting in increased inflammation. The increased inflammation occurs through the activation of multiple cell types including dendritic cells (DC). In this study, we investigated the activity of soluble products present in infected AD lesions by measuring the ability of patients' wash fluids from a quantitative culture of lesions to activate DC. We found that wash fluid derived from AD lesions induced cytokine production by murine bone marrow-derived DC, including IL-1β, IL-6, L-10, and tumor necrosis factor-α. The lipoprotein lipoteichoic acid (LTA) from Staphylococcus aureus was implicated as a potent stimulus in the wash fluids as only wash fluid samples that contained LTA exerted this activity, and exogenous LTA triggered similar DC cytokine activation. Wash fluid- and LTA-stimulated DC cytokine production required MyD88, but not the platelet-activating factor receptor (PAF-R), despite the ability of LTA to function through this receptor in keratinocytes. Thus, our results support a role for DC in the worsening of AD inflammation due to secondary bacteria infections.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalCellular Immunology
Volume267
Issue number1
DOIs
StatePublished - 2011

Fingerprint

Atopic Dermatitis
Dendritic Cells
Staphylococcus aureus
Cytokines
Inflammation
Coinfection
Interleukin-1
Keratinocytes
Lipoproteins
Interleukin-6
Tumor Necrosis Factor-alpha
Bone Marrow
lipoteichoic acid
Bacteria
Skin

Keywords

  • Atopic dermatitis
  • Dendritic cells
  • Interleukin-6
  • Lipoteichoic acid
  • Platelet-activating factor
  • Staphylococcus aureus
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Immunology

Cite this

Dendritic cells produce inflammatory cytokines in response to bacterial products from Staphylococcus aureus-infected atopic dermatitis lesions. / Voorhees, Timothy; Chang, Jihoon; Yao, Yongxue; Kaplan, Mark; Chang, Cheong Hee; Travers, Jeffrey.

In: Cellular Immunology, Vol. 267, No. 1, 2011, p. 17-22.

Research output: Contribution to journalArticle

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