Dental pulp stem cell responses to novel antibiotic-containing scaffolds for regenerative endodontics

K. Kamocki, J. E. Nör, M. C. Bottino

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Aim: To evaluate both the drug-release profile and the effects on human dental pulp stem cells' (hDPSC) proliferation and viability of novel bi-mix antibiotic-containing scaffolds intended for use as a drug delivery system for root canal disinfection prior to regenerative endodontics. Methodology: Polydioxanone (PDS)-based fibrous scaffolds containing both metronidazole (MET) and ciprofloxacin (CIP) at selected ratios were synthesized via electrospinning. Fibre diameter was evaluated based on scanning electron microscopy (SEM) images. Pure PDS scaffolds and a saturated CIP/MET solution (i.e. 50 mg of each antibiotic in 1 mL) (hereafter referred to as DAP) served as both negative (nontoxic) and positive (toxic) controls, respectively. High-performance liquid chromatography (HPLC) was performed to investigate the amount of drug(s) released from the scaffolds. WST-1® proliferation assay was used to evaluate the effect of the scaffolds on cell proliferation. LIVE/DEAD® assay was used to qualitatively assess cell viability. Data obtained from drug release and proliferation assays were statistically analysed at the 5% significance level. Results: A burst release of CIP and MET was noted within the first 24 h, followed by a sustained maintenance of the drug(s) concentration for 14 days. A concentration-dependent trend was noticed upon hDPSCs' exposure to all CIP-containing scaffolds, where increasing the CIP concentration resulted in reduced cell proliferation (P <0.05) and viability. In groups exposed to pure MET or pure PDS scaffolds, no changes in proliferation were observed. Conclusions: Synthesized antibiotic-containing scaffolds had significantly lower effects on hDPSCs proliferation when compared to the saturated CIP/MET solution (DAP).

Original languageEnglish (US)
Pages (from-to)1147-1156
Number of pages10
JournalInternational Endodontic Journal
Volume48
Issue number12
DOIs
StatePublished - Dec 1 2015
Externally publishedYes

Fingerprint

Dental Pulp
Endodontics
Ciprofloxacin
Metronidazole
Stem Cells
Anti-Bacterial Agents
Cell Proliferation
Cell Survival
Polydioxanone
Poisons
Dental Pulp Cavity
Disinfection
Drug Delivery Systems
Pharmaceutical Preparations
Electron Scanning Microscopy
High Pressure Liquid Chromatography
Maintenance

Keywords

  • Antibiotic pastes
  • Drug delivery
  • Nanofibres
  • Regeneration
  • Root canal
  • Stem cells

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Dental pulp stem cell responses to novel antibiotic-containing scaffolds for regenerative endodontics. / Kamocki, K.; Nör, J. E.; Bottino, M. C.

In: International Endodontic Journal, Vol. 48, No. 12, 01.12.2015, p. 1147-1156.

Research output: Contribution to journalArticle

Kamocki, K. ; Nör, J. E. ; Bottino, M. C. / Dental pulp stem cell responses to novel antibiotic-containing scaffolds for regenerative endodontics. In: International Endodontic Journal. 2015 ; Vol. 48, No. 12. pp. 1147-1156.
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