Dentine remineralisation by simulated saliva formulations with different Ca and Pi contents

Anderson Hara, R. L. Karlinsey, Domenick Zero

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The understanding of the dentine remineralisation process and the ability to reproduce it in vitro are essential to the development of preventive and therapeutic measures. This study investigated how simulated saliva formulations with different Ca and Pi contents and degrees of saturation with respect to biologically relevant calcium phosphates may affect the remineralisation of eroded dentine, as a function of time. Slabs of bovine root dentine (n = 8 per group) were flattened, polished, demineralised by 1% citric acid for 30 and 60 min and remineralised for 3, 7 and 14 days, by one of the following buffered (pH 7) solutions [Ca:Pi ratio, Ca/Pi concentrations (mM), ionic strength]: solution A: 1.6, 1.5/0.9, 0.115; solution B: 1.6, 2/1.25, 0.117; solution C: 1.6, 3.2/2, 0.121; solution D: 0.3, 1.11/3.7, 0.118; solution E: 0.3, 1.45/5, 0.122. Integrated mineral loss (30 and 60 min) was quantified by transverse microradiography after each remineralisation period. ANOVA and regression analyses (α = 0.05) showed, irrespective of the demineralisation time, that the solutions C and E were able to remineralise dentine. This effect increased throughout the remineralisation times and was significantly higher for E. Remineralisation was successfully shown in vitro, under specific conditions of degree of saturation and Ca and Pi contents of the solutions. Optimum remineralisation was observed for the solution E supersaturated with respect to relevant calcium phosphates, with low Ca:Pi ratio and highest Pi concentration.

Original languageEnglish (US)
Pages (from-to)51-56
Number of pages6
JournalCaries Research
Volume42
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Dentin
Saliva
Calcium Phosphates
Microradiography
Citric Acid
Osmolar Concentration
Minerals
Analysis of Variance
Regression Analysis

Keywords

  • Calcium
  • Dentine
  • Erosion
  • Phosphorus
  • Remineralisation

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Dentine remineralisation by simulated saliva formulations with different Ca and Pi contents. / Hara, Anderson; Karlinsey, R. L.; Zero, Domenick.

In: Caries Research, Vol. 42, No. 1, 01.2008, p. 51-56.

Research output: Contribution to journalArticle

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