### Abstract

The understanding of the dentine remineralisation process and the ability to reproduce it in vitro are essential to the development of preventive and therapeutic measures. This study investigated how simulated saliva formulations with different Ca and P_{i} contents and degrees of saturation with respect to biologically relevant calcium phosphates may affect the remineralisation of eroded dentine, as a function of time. Slabs of bovine root dentine (n = 8 per group) were flattened, polished, demineralised by 1% citric acid for 30 and 60 min and remineralised for 3, 7 and 14 days, by one of the following buffered (pH 7) solutions [Ca:P_{i} ratio, Ca/P_{i} concentrations (mM), ionic strength]: solution A: 1.6, 1.5/0.9, 0.115; solution B: 1.6, 2/1.25, 0.117; solution C: 1.6, 3.2/2, 0.121; solution D: 0.3, 1.11/3.7, 0.118; solution E: 0.3, 1.45/5, 0.122. Integrated mineral loss (30 and 60 min) was quantified by transverse microradiography after each remineralisation period. ANOVA and regression analyses (α = 0.05) showed, irrespective of the demineralisation time, that the solutions C and E were able to remineralise dentine. This effect increased throughout the remineralisation times and was significantly higher for E. Remineralisation was successfully shown in vitro, under specific conditions of degree of saturation and Ca and P_{i} contents of the solutions. Optimum remineralisation was observed for the solution E supersaturated with respect to relevant calcium phosphates, with low Ca:P_{i} ratio and highest P_{i} concentration.

Original language | English (US) |
---|---|

Pages (from-to) | 51-56 |

Number of pages | 6 |

Journal | Caries Research |

Volume | 42 |

Issue number | 1 |

DOIs | |

State | Published - Jan 2008 |

### Fingerprint

### Keywords

- Calcium
- Dentine
- Erosion
- Phosphorus
- Remineralisation

### ASJC Scopus subject areas

- Dentistry(all)

### Cite this

_{i}contents.

*Caries Research*,

*42*(1), 51-56. https://doi.org/10.1159/000111750

**Dentine remineralisation by simulated saliva formulations with different Ca and P _{i} contents.** / Hara, Anderson; Karlinsey, R. L.; Zero, Domenick.

Research output: Contribution to journal › Article

_{i}contents',

*Caries Research*, vol. 42, no. 1, pp. 51-56. https://doi.org/10.1159/000111750

_{i}contents. Caries Research. 2008 Jan;42(1):51-56. https://doi.org/10.1159/000111750

}

TY - JOUR

T1 - Dentine remineralisation by simulated saliva formulations with different Ca and Pi contents

AU - Hara, Anderson

AU - Karlinsey, R. L.

AU - Zero, Domenick

PY - 2008/1

Y1 - 2008/1

N2 - The understanding of the dentine remineralisation process and the ability to reproduce it in vitro are essential to the development of preventive and therapeutic measures. This study investigated how simulated saliva formulations with different Ca and Pi contents and degrees of saturation with respect to biologically relevant calcium phosphates may affect the remineralisation of eroded dentine, as a function of time. Slabs of bovine root dentine (n = 8 per group) were flattened, polished, demineralised by 1% citric acid for 30 and 60 min and remineralised for 3, 7 and 14 days, by one of the following buffered (pH 7) solutions [Ca:Pi ratio, Ca/Pi concentrations (mM), ionic strength]: solution A: 1.6, 1.5/0.9, 0.115; solution B: 1.6, 2/1.25, 0.117; solution C: 1.6, 3.2/2, 0.121; solution D: 0.3, 1.11/3.7, 0.118; solution E: 0.3, 1.45/5, 0.122. Integrated mineral loss (30 and 60 min) was quantified by transverse microradiography after each remineralisation period. ANOVA and regression analyses (α = 0.05) showed, irrespective of the demineralisation time, that the solutions C and E were able to remineralise dentine. This effect increased throughout the remineralisation times and was significantly higher for E. Remineralisation was successfully shown in vitro, under specific conditions of degree of saturation and Ca and Pi contents of the solutions. Optimum remineralisation was observed for the solution E supersaturated with respect to relevant calcium phosphates, with low Ca:Pi ratio and highest Pi concentration.

AB - The understanding of the dentine remineralisation process and the ability to reproduce it in vitro are essential to the development of preventive and therapeutic measures. This study investigated how simulated saliva formulations with different Ca and Pi contents and degrees of saturation with respect to biologically relevant calcium phosphates may affect the remineralisation of eroded dentine, as a function of time. Slabs of bovine root dentine (n = 8 per group) were flattened, polished, demineralised by 1% citric acid for 30 and 60 min and remineralised for 3, 7 and 14 days, by one of the following buffered (pH 7) solutions [Ca:Pi ratio, Ca/Pi concentrations (mM), ionic strength]: solution A: 1.6, 1.5/0.9, 0.115; solution B: 1.6, 2/1.25, 0.117; solution C: 1.6, 3.2/2, 0.121; solution D: 0.3, 1.11/3.7, 0.118; solution E: 0.3, 1.45/5, 0.122. Integrated mineral loss (30 and 60 min) was quantified by transverse microradiography after each remineralisation period. ANOVA and regression analyses (α = 0.05) showed, irrespective of the demineralisation time, that the solutions C and E were able to remineralise dentine. This effect increased throughout the remineralisation times and was significantly higher for E. Remineralisation was successfully shown in vitro, under specific conditions of degree of saturation and Ca and Pi contents of the solutions. Optimum remineralisation was observed for the solution E supersaturated with respect to relevant calcium phosphates, with low Ca:Pi ratio and highest Pi concentration.

KW - Calcium

KW - Dentine

KW - Erosion

KW - Phosphorus

KW - Remineralisation

UR - http://www.scopus.com/inward/record.url?scp=38349158381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38349158381&partnerID=8YFLogxK

U2 - 10.1159/000111750

DO - 10.1159/000111750

M3 - Article

VL - 42

SP - 51

EP - 56

JO - Caries Research

JF - Caries Research

SN - 0008-6568

IS - 1

ER -