Depletion of focal adhesion kinase by antisense depresses contractile activation of smooth muscle

Dale D. Tang, Susan J. Gunst

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Focal adhesion kinase (FAK) undergoes tyrosine phosphorylation in response to the contractile stimulation of tracheal smooth muscle. We hypothesized that FAK may play an important role in signaling pathways that regulate smooth muscle contraction. FAK antisense or FAK sense was introduced into muscle strips by reversible permeabilization, and strips were incubated with antisense or sense for 7 days. Antisense decreased FAK expression compared with that in untreated and sense-treated tissues, but it did not affect the expression of vinculin or myosin light chain kinase. Increases in force, intracellular free Ca2+, and myosin light chain phosphorylation in response to stimulation with ACh or KCl were depressed in FAK-depleted tissues, but FAK depletion did not affect the activation of permeabilized tracheal muscle strips with Ca2+. The tyrosine phosphorylation of paxillin, a substrate for FAK, was also significantly reduced in FAK-depleted strips. We conclude that FAK is a necessary component of the signaling pathways that regulate smooth muscle contraction and that FAK plays a role in regulating intracellular free Ca2+ and myosin light chain phosphorylation.

Original languageEnglish (US)
Pages (from-to)C874-C883
JournalAmerican Journal of Physiology - Cell Physiology
Volume280
Issue number4 49-4
StatePublished - Jun 5 2001

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Focal Adhesion Protein-Tyrosine Kinases
Smooth Muscle
Muscle
Chemical activation
Phosphorylation
Myosin Light Chains
Muscle Contraction
Tyrosine
Tissue Adhesions
Tissue
Paxillin
Vinculin
Myosin-Light-Chain Kinase
Muscles

Keywords

  • Cytoskeleton
  • Intracellular calcium
  • Myosin light chain phosphorylation
  • Paxillin
  • Smooth muscle contraction

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Depletion of focal adhesion kinase by antisense depresses contractile activation of smooth muscle. / Tang, Dale D.; Gunst, Susan J.

In: American Journal of Physiology - Cell Physiology, Vol. 280, No. 4 49-4, 05.06.2001, p. C874-C883.

Research output: Contribution to journalArticle

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