Depressed basal hypothalamic neuronal activity in type-1 diabetic mice is correlated with proinflammatory secretion of HMBG1

Jeffrey S. Thinschmidt, Luis M. Colon Perez, Marcelo Febo, Sergio Caballero, Michael A. King, Fletcher White, Maria B. Grant

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We recently found indicators of hypothalamic inflammation and neurodegeneration linked to the loss of neuroprotective factors including insulin-like growth factor (IGF-1) and IGF binding protein-2 (IGFBP-3) in mice made diabetic using streptozotocin (STZ). In the current work, a genetic model of type-1 diabetes (Ins2Akita mouse) was used to evaluate changes in neuronal activity and concomitant changes in the proinflammatory mediator high-mobility group box-1 (HMBG1). We found basal hypothalamic neuronal activity as indicated by manganese-enhanced magnetic resonance imaging (MEMRI) was significantly decreased in 8 months old, but not 2 months old Ins2Akita diabetic mice compared to controls. In tissue from the same animals we evaluated the expression of HMBG1 using immunohistochemistry and confocal microscopy. We found decreased HMBG1 nuclear localization in the paraventricular nucleus of the hypothalamus (PVN) in 8 months old, but not 2 months old diabetic animals indicating nuclear release of the protein consistent with an inflammatory state. Adjacent thalamic regions showed little change in HMBG1 nuclear localization and neuronal activity as a result of diabetes. This work extends our previous findings demonstrating changes consistent with hypothalamic neuroinflammation in STZ treated animals, and shows active inflammatory processes are correlated with changes in basal hypothalamic neuronal activity in Ins2Akita mice.

Original languageEnglish (US)
Pages (from-to)21-27
Number of pages7
JournalNeuroscience Letters
Volume615
DOIs
StatePublished - Feb 26 2016

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Streptozocin
Insulin-Like Growth Factor Binding Protein 2
Insulin-Like Growth Factor Binding Protein 3
Paraventricular Hypothalamic Nucleus
Genetic Models
Somatomedins
Manganese
Nuclear Proteins
Type 1 Diabetes Mellitus
Insulin-Like Growth Factor I
Confocal Microscopy
Hypothalamus
Immunohistochemistry
Magnetic Resonance Imaging
Inflammation

Keywords

  • Akita
  • Diabetes
  • HMBG1
  • MEMRI
  • Neuroinflammation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Depressed basal hypothalamic neuronal activity in type-1 diabetic mice is correlated with proinflammatory secretion of HMBG1. / Thinschmidt, Jeffrey S.; Colon Perez, Luis M.; Febo, Marcelo; Caballero, Sergio; King, Michael A.; White, Fletcher; Grant, Maria B.

In: Neuroscience Letters, Vol. 615, 26.02.2016, p. 21-27.

Research output: Contribution to journalArticle

Thinschmidt, Jeffrey S. ; Colon Perez, Luis M. ; Febo, Marcelo ; Caballero, Sergio ; King, Michael A. ; White, Fletcher ; Grant, Maria B. / Depressed basal hypothalamic neuronal activity in type-1 diabetic mice is correlated with proinflammatory secretion of HMBG1. In: Neuroscience Letters. 2016 ; Vol. 615. pp. 21-27.
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