Dermal lymphatic dilation in a mouse model of alopecia areata

John P. Sundberg, C. Herbert Pratt, Kathleen A. Silva, Victoria E. Kennedy, Timothy M. Stearns, Beth A. Sundberg, Lloyd E. King, Harm HogenEsch

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mouse models of various types of inflammatory skin disease are often accompanied by increased dermal angiogenesis. The C3H/HeJ inbred strain spontaneously develops alopecia areata (AA), a cell mediated autoimmune disorder that can be controllably expanded using full thickness skin grafts to young unaffected mice. This provides a reproducible and progressive model for AA in which the vascularization of the skin can be examined. Mice receiving skin grafts from AA or normal mice were evaluated at 5, 10, 15, and 20 weeks after engraftment. Lymphatics are often overlooked as they are small slit-like structures above the hair follicle that resemble artifact-like separation of collagen bundles with some fixatives. Lymphatics are easily detected using lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1) by immunohistochemistry to label their endothelial cells. Using LYVE1, there were no changes in distribution or numbers of lymphatics although they were more prominent (dilated) in the mice with AA. Lyve1 transcripts were not significantly upregulated except at 10 weeks after skin grafting when clinical signs of AA first become apparent. Other genes involved with vascular growth and dilation or movement of immune cells were dysregulated, mostly upregulated. These findings emphasize aspects of AA not commonly considered and provide potential targets for therapeutic intervention.

Original languageEnglish (US)
Pages (from-to)332-336
Number of pages5
JournalExperimental and Molecular Pathology
Volume100
Issue number2
DOIs
StatePublished - Apr 1 2016
Externally publishedYes

Fingerprint

Alopecia Areata
Dilatation
Skin
CD44 Antigens
Lymphatic Vessels
Grafts
Fixatives
Endothelial cells
Transplants
Skin Transplantation
Hair Follicle
Labels
Skin Diseases
Collagen
Artifacts
Genes
Cell Movement
Blood Vessels
Endothelial Cells
Immunohistochemistry

Keywords

  • Autoimmune disease
  • Inflammation
  • Lymphatics
  • LYVE1
  • PECAM1
  • SMA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Sundberg, J. P., Pratt, C. H., Silva, K. A., Kennedy, V. E., Stearns, T. M., Sundberg, B. A., ... HogenEsch, H. (2016). Dermal lymphatic dilation in a mouse model of alopecia areata. Experimental and Molecular Pathology, 100(2), 332-336. https://doi.org/10.1016/j.yexmp.2016.03.001

Dermal lymphatic dilation in a mouse model of alopecia areata. / Sundberg, John P.; Pratt, C. Herbert; Silva, Kathleen A.; Kennedy, Victoria E.; Stearns, Timothy M.; Sundberg, Beth A.; King, Lloyd E.; HogenEsch, Harm.

In: Experimental and Molecular Pathology, Vol. 100, No. 2, 01.04.2016, p. 332-336.

Research output: Contribution to journalArticle

Sundberg, JP, Pratt, CH, Silva, KA, Kennedy, VE, Stearns, TM, Sundberg, BA, King, LE & HogenEsch, H 2016, 'Dermal lymphatic dilation in a mouse model of alopecia areata', Experimental and Molecular Pathology, vol. 100, no. 2, pp. 332-336. https://doi.org/10.1016/j.yexmp.2016.03.001
Sundberg JP, Pratt CH, Silva KA, Kennedy VE, Stearns TM, Sundberg BA et al. Dermal lymphatic dilation in a mouse model of alopecia areata. Experimental and Molecular Pathology. 2016 Apr 1;100(2):332-336. https://doi.org/10.1016/j.yexmp.2016.03.001
Sundberg, John P. ; Pratt, C. Herbert ; Silva, Kathleen A. ; Kennedy, Victoria E. ; Stearns, Timothy M. ; Sundberg, Beth A. ; King, Lloyd E. ; HogenEsch, Harm. / Dermal lymphatic dilation in a mouse model of alopecia areata. In: Experimental and Molecular Pathology. 2016 ; Vol. 100, No. 2. pp. 332-336.
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