Description of human AAA by cytokine and immune cell aberrations compared to risk-factor matched controls

S. Keisin Wang, Linden A. Green, Ashley R. Gutwein, Natalie A. Drucker, Raghu Motaganahalli, Alok K. Gupta, Andres Fajardo, Michael Murphy

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: The pathogenesis driving the formation of abdominal aortic aneurysms continues to be poorly understood. Therefore, we systemically define the cytokine and circulating immune cell environment observed in human abdominal aortic aneurysm compared with risk-factor matched controls. Methods: From 2015 to 2017, a total of 274 patients donated blood to the Indiana University Center for Aortic Disease. Absolute concentrations of circulating cytokines were determined, using enzyme-linked immunosorbent assays while the expression of circulating immune cell phenotypes were assayed via flow cytometric analysis. Results: Human abdominal aortic aneurysm is characterized by a significant depletion of the antigen-specific, CD4+ Tr1 regulatory lymphocyte that corresponds to an upregulation of the antigen-specific, inflammatory Th17 cell. We found no differences in the incidence of Treg, B10, and myeloid-derived suppressor regulatory cells. Similarly, no disparities were noted in the following inflammatory cytokines: IL-1β C-reactive protein, tumor necrosis factor α interferon γ and IL-23. However, significant upregulation of the inflammatory cytokines osteopontin, IL-6, and IL-17 were noted. Additionally, no changes were observed in the regulatory cytokines IL-2, IL-4, IL-13, TNF-stimulated gene 6 protein, and prostaglandin E2, but we did observe a significant decrease in the essential regulatory cytokine IL-10. Conclusion: In this investigation, we systematically characterize the abdominal aortic aneurysm–immune environment and present preliminary evidence that faulty immune regulation may also contribute to aneurysm formation and growth.

Original languageEnglish (US)
JournalSurgery (United States)
DOIs
StateAccepted/In press - Jan 1 2018

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Cytokines
Abdominal Aortic Aneurysm
Up-Regulation
Interleukin-23
Th17 Cells
Aortic Diseases
CD4 Antigens
Osteopontin
Interleukin-13
Interleukin-17
Interleukin-1
Dinoprostone
Interleukin-4
Interleukin-10
C-Reactive Protein
Interferons
Interleukin-2
Aneurysm
Interleukin-6
Tumor Necrosis Factor-alpha

ASJC Scopus subject areas

  • Surgery

Cite this

Description of human AAA by cytokine and immune cell aberrations compared to risk-factor matched controls. / Wang, S. Keisin; Green, Linden A.; Gutwein, Ashley R.; Drucker, Natalie A.; Motaganahalli, Raghu; Gupta, Alok K.; Fajardo, Andres; Murphy, Michael.

In: Surgery (United States), 01.01.2018.

Research output: Contribution to journalArticle

Wang, S. Keisin ; Green, Linden A. ; Gutwein, Ashley R. ; Drucker, Natalie A. ; Motaganahalli, Raghu ; Gupta, Alok K. ; Fajardo, Andres ; Murphy, Michael. / Description of human AAA by cytokine and immune cell aberrations compared to risk-factor matched controls. In: Surgery (United States). 2018.
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