Desmoglein-1 is a minor autoantigen in dogs with pemphigus foliaceus

Thierry Olivry, Alora LaVoy, Stanley M. Dunston, Rachel S. Brown, Elizabeth M. Lennon, Simon Warren, Phillip Prisayanh, Eliane J. Müller, Maja M. Suter, Gregg A. Dean

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The majority of human patients with pemphigus foliaceus (PF) have circulating IgG autoantibodies that target conformational epitopes on the desmosomal cadherin desmoglein-1 (dsg1). Limited studies using immunoblot techniques suggested that the principal autoantigen in dogs with PF might also be dsg1. It was the objective of this study to test this hypothesis. A comprehensive survey of canine PF sera was conducted using a novel screening strategy that detects conformational epitopes. This method consists of the ectopic expression of canine dsg1 at the surface of human 293T epithelial kidney cells and their live screening, i.e. prior to fixation. Out of seven control human PF sera that bound to canine epidermis, three (57%) contained IgG autoantibodies that recognized ectopically expressed canine dsg1 with a membrane and punctate pattern. Out of 83 canine PF sera only five (6%) contained IgG that recognized canine dsg1. Consistent with findings for human PF sera obtained in this study, autoantibody binding was conformation- and glycosylation- dependent as demonstrated by calcium chelation with EDTA and tunicamycin or wheat germ agglutinin treatment, respectively. In conclusion, these studies establish canine dsg1 as a minor autoantigen for canine PF. Antigenic epitopes appear to be conformation- and glycosylation-dependent.

Original languageEnglish (US)
Pages (from-to)245-255
Number of pages11
JournalVeterinary Immunology and Immunopathology
Volume110
Issue number3-4
DOIs
StatePublished - Apr 15 2006
Externally publishedYes

Fingerprint

Desmoglein 1
autoantigens
Pemphigus
Autoantigens
Canidae
Dogs
dogs
autoantibodies
Autoantibodies
Epitopes
epitopes
Immunoglobulin G
Serum
Glycosylation
glycosylation
Desmosomal Cadherins
Tunicamycin
Wheat Germ Agglutinins
screening
tunicamycin

Keywords

  • Autoimmunity
  • Blistering
  • Cadherin
  • Canine
  • Desmosome
  • Skin

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Olivry, T., LaVoy, A., Dunston, S. M., Brown, R. S., Lennon, E. M., Warren, S., ... Dean, G. A. (2006). Desmoglein-1 is a minor autoantigen in dogs with pemphigus foliaceus. Veterinary Immunology and Immunopathology, 110(3-4), 245-255. https://doi.org/10.1016/j.vetimm.2005.10.002

Desmoglein-1 is a minor autoantigen in dogs with pemphigus foliaceus. / Olivry, Thierry; LaVoy, Alora; Dunston, Stanley M.; Brown, Rachel S.; Lennon, Elizabeth M.; Warren, Simon; Prisayanh, Phillip; Müller, Eliane J.; Suter, Maja M.; Dean, Gregg A.

In: Veterinary Immunology and Immunopathology, Vol. 110, No. 3-4, 15.04.2006, p. 245-255.

Research output: Contribution to journalArticle

Olivry, T, LaVoy, A, Dunston, SM, Brown, RS, Lennon, EM, Warren, S, Prisayanh, P, Müller, EJ, Suter, MM & Dean, GA 2006, 'Desmoglein-1 is a minor autoantigen in dogs with pemphigus foliaceus', Veterinary Immunology and Immunopathology, vol. 110, no. 3-4, pp. 245-255. https://doi.org/10.1016/j.vetimm.2005.10.002
Olivry, Thierry ; LaVoy, Alora ; Dunston, Stanley M. ; Brown, Rachel S. ; Lennon, Elizabeth M. ; Warren, Simon ; Prisayanh, Phillip ; Müller, Eliane J. ; Suter, Maja M. ; Dean, Gregg A. / Desmoglein-1 is a minor autoantigen in dogs with pemphigus foliaceus. In: Veterinary Immunology and Immunopathology. 2006 ; Vol. 110, No. 3-4. pp. 245-255.
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