Detection of an IE responsive element(s) in the BamHI J fragment of human cytomegalovirus AD169

Anne E. Reifel-Miller, Chao-Hung Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The BamHI J fragment of human cytomegalovirus (HCMV) AD169 located at 0.815 to 0.855 map units in the unique short component of the genome was demonstrated to be responsive to the HCMV IE proteins by using a transient chloramphenicol acetyltransferase (cat) gene expression system. The BamHI J fragment was cloned into a cat gene expression plasmid and then cotransfected with a plasmid that expresses the immediate early (IE) genes of HCMV AD169 into the HCMV permissive cell line MRC-5. The results indicated that the BamHl 1 fragment enhanced cat gene expression 10-fold when the HCMV IE proteins were present. The BamHI J fragment was demonstrated to have properties of an inducible enhancer. In the presence of the HCMV IE proteins, it enhances cat gene expression when positioned in either orientation both upstream and downstream from the cat gene; it enhances transcription from the herpes simplex virus type 1 (HSV-1) thymidine kinase gene and the simian virus 40 (SV40) early gene promoters; and it requires a cis-positioned promoter for enhancer activity.

Original languageEnglish
Pages (from-to)496-504
Number of pages9
JournalVirology
Volume177
Issue number2
DOIs
StatePublished - 1990

Fingerprint

Chloramphenicol O-Acetyltransferase
Cytomegalovirus
Gene Expression
Plasmids
Genome Components
Genes
Immediate-Early Genes
Simian virus 40
Thymidine Kinase
Human Herpesvirus 1
Cell Line
cytomegalovirus immediate-early proteins

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Detection of an IE responsive element(s) in the BamHI J fragment of human cytomegalovirus AD169. / Reifel-Miller, Anne E.; Lee, Chao-Hung.

In: Virology, Vol. 177, No. 2, 1990, p. 496-504.

Research output: Contribution to journalArticle

Reifel-Miller, Anne E. ; Lee, Chao-Hung. / Detection of an IE responsive element(s) in the BamHI J fragment of human cytomegalovirus AD169. In: Virology. 1990 ; Vol. 177, No. 2. pp. 496-504.
@article{1d0a2d6c11a6450ba984672d521554dc,
title = "Detection of an IE responsive element(s) in the BamHI J fragment of human cytomegalovirus AD169",
abstract = "The BamHI J fragment of human cytomegalovirus (HCMV) AD169 located at 0.815 to 0.855 map units in the unique short component of the genome was demonstrated to be responsive to the HCMV IE proteins by using a transient chloramphenicol acetyltransferase (cat) gene expression system. The BamHI J fragment was cloned into a cat gene expression plasmid and then cotransfected with a plasmid that expresses the immediate early (IE) genes of HCMV AD169 into the HCMV permissive cell line MRC-5. The results indicated that the BamHl 1 fragment enhanced cat gene expression 10-fold when the HCMV IE proteins were present. The BamHI J fragment was demonstrated to have properties of an inducible enhancer. In the presence of the HCMV IE proteins, it enhances cat gene expression when positioned in either orientation both upstream and downstream from the cat gene; it enhances transcription from the herpes simplex virus type 1 (HSV-1) thymidine kinase gene and the simian virus 40 (SV40) early gene promoters; and it requires a cis-positioned promoter for enhancer activity.",
author = "Reifel-Miller, {Anne E.} and Chao-Hung Lee",
year = "1990",
doi = "10.1016/0042-6822(90)90514-R",
language = "English",
volume = "177",
pages = "496--504",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Detection of an IE responsive element(s) in the BamHI J fragment of human cytomegalovirus AD169

AU - Reifel-Miller, Anne E.

AU - Lee, Chao-Hung

PY - 1990

Y1 - 1990

N2 - The BamHI J fragment of human cytomegalovirus (HCMV) AD169 located at 0.815 to 0.855 map units in the unique short component of the genome was demonstrated to be responsive to the HCMV IE proteins by using a transient chloramphenicol acetyltransferase (cat) gene expression system. The BamHI J fragment was cloned into a cat gene expression plasmid and then cotransfected with a plasmid that expresses the immediate early (IE) genes of HCMV AD169 into the HCMV permissive cell line MRC-5. The results indicated that the BamHl 1 fragment enhanced cat gene expression 10-fold when the HCMV IE proteins were present. The BamHI J fragment was demonstrated to have properties of an inducible enhancer. In the presence of the HCMV IE proteins, it enhances cat gene expression when positioned in either orientation both upstream and downstream from the cat gene; it enhances transcription from the herpes simplex virus type 1 (HSV-1) thymidine kinase gene and the simian virus 40 (SV40) early gene promoters; and it requires a cis-positioned promoter for enhancer activity.

AB - The BamHI J fragment of human cytomegalovirus (HCMV) AD169 located at 0.815 to 0.855 map units in the unique short component of the genome was demonstrated to be responsive to the HCMV IE proteins by using a transient chloramphenicol acetyltransferase (cat) gene expression system. The BamHI J fragment was cloned into a cat gene expression plasmid and then cotransfected with a plasmid that expresses the immediate early (IE) genes of HCMV AD169 into the HCMV permissive cell line MRC-5. The results indicated that the BamHl 1 fragment enhanced cat gene expression 10-fold when the HCMV IE proteins were present. The BamHI J fragment was demonstrated to have properties of an inducible enhancer. In the presence of the HCMV IE proteins, it enhances cat gene expression when positioned in either orientation both upstream and downstream from the cat gene; it enhances transcription from the herpes simplex virus type 1 (HSV-1) thymidine kinase gene and the simian virus 40 (SV40) early gene promoters; and it requires a cis-positioned promoter for enhancer activity.

UR - http://www.scopus.com/inward/record.url?scp=0025344880&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025344880&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(90)90514-R

DO - 10.1016/0042-6822(90)90514-R

M3 - Article

C2 - 2164722

AN - SCOPUS:0025344880

VL - 177

SP - 496

EP - 504

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -