Detection of antibodies against streptococcus mutans cell wall contaminating sera against ribosomal fractions

Richard Gregory, Isaac L. Shechmeister

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A modification of the immunoprecipitation technique was developed to precipitate ribosomal proteins from Streptococcus mutans in isoelectric focusing gels. The method was established in order to determine whether the ribosomal immunogen was contaminated with cell surface proteins. Isoelectric focusing was used to separate the proteins into bands in the gel and various antisera utilized to precipitate the antigens. Electrophoresis of the ribosomal preparation produced 19 protein bands. A rat antiserum raised against the preparation reacted with 8 of these bands and an aliquot of the same antiserum which had been adsorbed with whole S. mutans cells reacted with only 6 bands. This procedure allowed us to detect contamination of the ribosomal preparation with cell surface antigens. The method may be used to detect contamination with cell surface antigens in many different subcellular preparations.

Original languageEnglish (US)
Pages (from-to)141-145
Number of pages5
JournalJournal of Immunological Methods
Volume60
Issue number1-2
DOIs
StatePublished - May 27 1983
Externally publishedYes

Fingerprint

Streptococcus mutans
Cell Wall
Immune Sera
Isoelectric Focusing
Surface Antigens
Antibodies
Gels
Serum
Ribosomal Proteins
Immunoprecipitation
Electrophoresis
Membrane Proteins
Proteins
Antigens

Keywords

  • cell wall antigens
  • ribosomal antigens
  • Streptococcus mutans antisera

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Detection of antibodies against streptococcus mutans cell wall contaminating sera against ribosomal fractions. / Gregory, Richard; Shechmeister, Isaac L.

In: Journal of Immunological Methods, Vol. 60, No. 1-2, 27.05.1983, p. 141-145.

Research output: Contribution to journalArticle

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