Detection of apoptosis in weaver cerebellum by electron microscopic in situ end-labeling of fragmented DNA

Antonio Migheli, Angelo Attanasio, Wei Hua Lee, Shirley A. Bayer, Bernardino Ghetti

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Massive degeneration of granule cell precursors occurs perinatally in the cerebellum of weaver mutant mice. We have studied the electron microscopic (EM) features of granule cell death in weaver and control mice, using an in situ end-labeling (ISEL) technique for detecting DNA fragmentation, a hallmark of apoptosis. In all animals, EM-ISEL revealed the pattern of apoptosis, with an enhanced expression in weaver mice. The weaver gene appears to accelerate the death program, most likely through potassium channel-mediated signals.

Original languageEnglish
Pages (from-to)53-56
Number of pages4
JournalNeuroscience Letters
Volume199
Issue number1
DOIs
StatePublished - Oct 13 1995

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Neurologic Mutant Mice
Cerebellum
Electrons
Apoptosis
DNA
Potassium Channels
DNA Fragmentation
Cell Death
Genes

Keywords

  • Apoptosis
  • Development
  • DNA fragmentation
  • Electron microscopy
  • In situ end-labeling
  • Weaver

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Detection of apoptosis in weaver cerebellum by electron microscopic in situ end-labeling of fragmented DNA. / Migheli, Antonio; Attanasio, Angelo; Lee, Wei Hua; Bayer, Shirley A.; Ghetti, Bernardino.

In: Neuroscience Letters, Vol. 199, No. 1, 13.10.1995, p. 53-56.

Research output: Contribution to journalArticle

Migheli, Antonio ; Attanasio, Angelo ; Lee, Wei Hua ; Bayer, Shirley A. ; Ghetti, Bernardino. / Detection of apoptosis in weaver cerebellum by electron microscopic in situ end-labeling of fragmented DNA. In: Neuroscience Letters. 1995 ; Vol. 199, No. 1. pp. 53-56.
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