Detection of apoptosis in weaver cerebellum by electron microscopic in situ end-labeling of fragmented DNA

Antonio Migheli, Angelo Attanasio, Wei Hua Lee, Shirley A. Bayer, Bernardino Ghetti

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Massive degeneration of granule cell precursors occurs perinatally in the cerebellum of weaver mutant mice. We have studied the electron microscopic (EM) features of granule cell death in weaver and control mice, using an in situ end-labeling (ISEL) technique for detecting DNA fragmentation, a hallmark of apoptosis. In all animals, EM-ISEL revealed the pattern of apoptosis, with an enhanced expression in weaver mice. The weaver gene appears to accelerate the death program, most likely through potassium channel-mediated signals.

Original languageEnglish (US)
Pages (from-to)53-56
Number of pages4
JournalNeuroscience Letters
Volume199
Issue number1
DOIs
StatePublished - Oct 13 1995

Keywords

  • Apoptosis
  • Development
  • DNA fragmentation
  • Electron microscopy
  • In situ end-labeling
  • Weaver

ASJC Scopus subject areas

  • Neuroscience(all)

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