Detection of apoptosis of bone cells in vitro

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Studies during the last decade demonstrated that apoptosis is as important as mitosis for the growth and maintenance of the skeleton and provided information on the significance and molecular regulation of apoptosis of bone cells. It is now known that: (1) all osteoclasts die by apoptosis after completing a bone resorption cycle; (2) the majority of osteoblasts also die, whereas the remainder become lining cells or osteocytes; and (3) osteocytes, although long-living cells, also can die prematurely. Furthermore, mounting evidence indicates that systemic hormones, local growth factors, cytokines, and pharmacological agents, as well as mechanical forces regulate the rate of bone cell apoptosis. This chapter summarizes the methods developed in the last few years to examine apoptosis of cultured bone cells and identify the signaling pathways and molecules involved in apoptosis regulation by diverse skeletal stimuli.

Original languageEnglish (US)
Title of host publicationOsteoporosis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages51-75
Number of pages25
ISBN (Print)9781588298287
DOIs
StatePublished - Jan 1 2008

Publication series

NameMethods in Molecular Biology
Volume455
ISSN (Print)1064-3745

Fingerprint

Apoptosis
Bone and Bones
Osteocytes
Osteoclasts
Bone Resorption
Osteoblasts
Mitosis
Skeleton
In Vitro Techniques
Cultured Cells
Intercellular Signaling Peptides and Proteins
Maintenance
Hormones
Pharmacology
Cytokines
Growth

Keywords

  • Apoptosis
  • Bone
  • Osteoblasts
  • Osteoclasts
  • Osteocytes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bellido, T., & Plotkin, L. I. (2008). Detection of apoptosis of bone cells in vitro. In Osteoporosis: Methods and Protocols (pp. 51-75). (Methods in Molecular Biology; Vol. 455). Humana Press. https://doi.org/10.1007/978-1-59745-104-8_4

Detection of apoptosis of bone cells in vitro. / Bellido, Teresita; Plotkin, Lilian I.

Osteoporosis: Methods and Protocols. Humana Press, 2008. p. 51-75 (Methods in Molecular Biology; Vol. 455).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bellido, T & Plotkin, LI 2008, Detection of apoptosis of bone cells in vitro. in Osteoporosis: Methods and Protocols. Methods in Molecular Biology, vol. 455, Humana Press, pp. 51-75. https://doi.org/10.1007/978-1-59745-104-8_4
Bellido T, Plotkin LI. Detection of apoptosis of bone cells in vitro. In Osteoporosis: Methods and Protocols. Humana Press. 2008. p. 51-75. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-104-8_4
Bellido, Teresita ; Plotkin, Lilian I. / Detection of apoptosis of bone cells in vitro. Osteoporosis: Methods and Protocols. Humana Press, 2008. pp. 51-75 (Methods in Molecular Biology).
@inbook{587f87213a504e8aa4d49ccff5d70190,
title = "Detection of apoptosis of bone cells in vitro",
abstract = "Studies during the last decade demonstrated that apoptosis is as important as mitosis for the growth and maintenance of the skeleton and provided information on the significance and molecular regulation of apoptosis of bone cells. It is now known that: (1) all osteoclasts die by apoptosis after completing a bone resorption cycle; (2) the majority of osteoblasts also die, whereas the remainder become lining cells or osteocytes; and (3) osteocytes, although long-living cells, also can die prematurely. Furthermore, mounting evidence indicates that systemic hormones, local growth factors, cytokines, and pharmacological agents, as well as mechanical forces regulate the rate of bone cell apoptosis. This chapter summarizes the methods developed in the last few years to examine apoptosis of cultured bone cells and identify the signaling pathways and molecules involved in apoptosis regulation by diverse skeletal stimuli.",
keywords = "Apoptosis, Bone, Osteoblasts, Osteoclasts, Osteocytes",
author = "Teresita Bellido and Plotkin, {Lilian I.}",
year = "2008",
month = "1",
day = "1",
doi = "10.1007/978-1-59745-104-8_4",
language = "English (US)",
isbn = "9781588298287",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "51--75",
booktitle = "Osteoporosis",

}

TY - CHAP

T1 - Detection of apoptosis of bone cells in vitro

AU - Bellido, Teresita

AU - Plotkin, Lilian I.

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Studies during the last decade demonstrated that apoptosis is as important as mitosis for the growth and maintenance of the skeleton and provided information on the significance and molecular regulation of apoptosis of bone cells. It is now known that: (1) all osteoclasts die by apoptosis after completing a bone resorption cycle; (2) the majority of osteoblasts also die, whereas the remainder become lining cells or osteocytes; and (3) osteocytes, although long-living cells, also can die prematurely. Furthermore, mounting evidence indicates that systemic hormones, local growth factors, cytokines, and pharmacological agents, as well as mechanical forces regulate the rate of bone cell apoptosis. This chapter summarizes the methods developed in the last few years to examine apoptosis of cultured bone cells and identify the signaling pathways and molecules involved in apoptosis regulation by diverse skeletal stimuli.

AB - Studies during the last decade demonstrated that apoptosis is as important as mitosis for the growth and maintenance of the skeleton and provided information on the significance and molecular regulation of apoptosis of bone cells. It is now known that: (1) all osteoclasts die by apoptosis after completing a bone resorption cycle; (2) the majority of osteoblasts also die, whereas the remainder become lining cells or osteocytes; and (3) osteocytes, although long-living cells, also can die prematurely. Furthermore, mounting evidence indicates that systemic hormones, local growth factors, cytokines, and pharmacological agents, as well as mechanical forces regulate the rate of bone cell apoptosis. This chapter summarizes the methods developed in the last few years to examine apoptosis of cultured bone cells and identify the signaling pathways and molecules involved in apoptosis regulation by diverse skeletal stimuli.

KW - Apoptosis

KW - Bone

KW - Osteoblasts

KW - Osteoclasts

KW - Osteocytes

UR - http://www.scopus.com/inward/record.url?scp=84934436722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934436722&partnerID=8YFLogxK

U2 - 10.1007/978-1-59745-104-8_4

DO - 10.1007/978-1-59745-104-8_4

M3 - Chapter

C2 - 18463810

AN - SCOPUS:84934436722

SN - 9781588298287

T3 - Methods in Molecular Biology

SP - 51

EP - 75

BT - Osteoporosis

PB - Humana Press

ER -