Detection of apoptosis of bone cells in vitro

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Studies during the last decade demonstrated that apoptosis is as important as mitosis for the growth and maintenance of the skeleton and provided information on the significance and molecular regulation of apoptosis of bone cells. It is now known that: (1) all osteoclasts die by apoptosis after completing a bone resorption cycle; (2) the majority of osteoblasts also die, whereas the remainder become lining cells or osteocytes; and (3) osteocytes, although long-living cells, also can die prematurely. Furthermore, mounting evidence indicates that systemic hormones, local growth factors, cytokines, and pharmacological agents, as well as mechanical forces regulate the rate of bone cell apoptosis. This chapter summarizes the methods developed in the last few years to examine apoptosis of cultured bone cells and identify the signaling pathways and molecules involved in apoptosis regulation by diverse skeletal stimuli.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages51-75
Number of pages25
Volume455
ISBN (Print)9781588298287
DOIs
StatePublished - 2008
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume455
ISSN (Print)10643745

Fingerprint

Apoptosis
Bone and Bones
Osteocytes
Osteoclasts
Bone Resorption
Osteoblasts
Mitosis
Skeleton
In Vitro Techniques
Cultured Cells
Intercellular Signaling Peptides and Proteins
Maintenance
Hormones
Pharmacology
Cytokines
Growth

Keywords

  • Apoptosis
  • Bone
  • Osteoblasts
  • Osteoclasts
  • Osteocytes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bellido, T., & Plotkin, L. (2008). Detection of apoptosis of bone cells in vitro. In Methods in Molecular Biology (Vol. 455, pp. 51-75). (Methods in Molecular Biology; Vol. 455). Humana Press. https://doi.org/10.1007/978-1-59745-104-8_4

Detection of apoptosis of bone cells in vitro. / Bellido, Teresita; Plotkin, Lilian.

Methods in Molecular Biology. Vol. 455 Humana Press, 2008. p. 51-75 (Methods in Molecular Biology; Vol. 455).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bellido, T & Plotkin, L 2008, Detection of apoptosis of bone cells in vitro. in Methods in Molecular Biology. vol. 455, Methods in Molecular Biology, vol. 455, Humana Press, pp. 51-75. https://doi.org/10.1007/978-1-59745-104-8_4
Bellido T, Plotkin L. Detection of apoptosis of bone cells in vitro. In Methods in Molecular Biology. Vol. 455. Humana Press. 2008. p. 51-75. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-104-8_4
Bellido, Teresita ; Plotkin, Lilian. / Detection of apoptosis of bone cells in vitro. Methods in Molecular Biology. Vol. 455 Humana Press, 2008. pp. 51-75 (Methods in Molecular Biology).
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