Detection of BRAF mutations on direct smears of thyroid fine-needle aspirates through cell transfer technique

Qiuying Shi, Ashley Ibrahim, Kristi Herbert, Marcia Carvin, Melissa Randolph, Kristin M. Post, Kendra Curless, Shaoxiong Chen, Harvey Cramer, Liang Cheng, Howard Wu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

OBJECTIVES: To determine the utility of the cell transfer technique (CTT) for BRAF molecular testing on thyroid fine-needle aspiration (FNA) specimens.

METHODS: Polymerase chain reaction (PCR)-based BRAF molecular testing was performed on tissues obtained through CTT from both air-dried and ethanol-fixed direct smears of thyroid FNA specimens and then compared with the corresponding thyroidectomy formalin-fixed, paraffin-embedded (FFPE) tissues on 30 cases.

RESULTS: BRAF testing was successfully performed on 29 of 30 air-dried CTT, 27 of 30 ethanol-fixed CTT, and 27 of 30 FFPE tissues. The results exhibited 11, 13, and 13 BRAF mutations and 18, 14, and 14 wild types for the air-dried CTT, the ethanol-fixed CTT, and the FFPE tissues, respectively. The concordance rate was 96% between air-dried and ethanol-fixed CTT tissues, 88% between air-dried CTT and FFPE tissues, and 92% between ethanol-fixed CTT and FFPE tissues.

CONCLUSIONS: PCR-based BRAF mutational testing can be reliably performed on the direct smears of the thyroid FNA specimens through the application of CTT.

Original languageEnglish
Pages (from-to)500-504
Number of pages5
JournalAmerican Journal of Clinical Pathology
Volume143
Issue number4
DOIs
StatePublished - Apr 1 2015

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Needles
Thyroid Gland
Mutation
Paraffin
Formaldehyde
Ethanol
Air
Fine Needle Biopsy
Polymerase Chain Reaction
Thyroidectomy

Keywords

  • BRAF mutation
  • Cell transfer
  • Cytology
  • Molecular
  • Thyroid

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Detection of BRAF mutations on direct smears of thyroid fine-needle aspirates through cell transfer technique. / Shi, Qiuying; Ibrahim, Ashley; Herbert, Kristi; Carvin, Marcia; Randolph, Melissa; Post, Kristin M.; Curless, Kendra; Chen, Shaoxiong; Cramer, Harvey; Cheng, Liang; Wu, Howard.

In: American Journal of Clinical Pathology, Vol. 143, No. 4, 01.04.2015, p. 500-504.

Research output: Contribution to journalArticle

Shi, Qiuying ; Ibrahim, Ashley ; Herbert, Kristi ; Carvin, Marcia ; Randolph, Melissa ; Post, Kristin M. ; Curless, Kendra ; Chen, Shaoxiong ; Cramer, Harvey ; Cheng, Liang ; Wu, Howard. / Detection of BRAF mutations on direct smears of thyroid fine-needle aspirates through cell transfer technique. In: American Journal of Clinical Pathology. 2015 ; Vol. 143, No. 4. pp. 500-504.
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AB - OBJECTIVES: To determine the utility of the cell transfer technique (CTT) for BRAF molecular testing on thyroid fine-needle aspiration (FNA) specimens.METHODS: Polymerase chain reaction (PCR)-based BRAF molecular testing was performed on tissues obtained through CTT from both air-dried and ethanol-fixed direct smears of thyroid FNA specimens and then compared with the corresponding thyroidectomy formalin-fixed, paraffin-embedded (FFPE) tissues on 30 cases.RESULTS: BRAF testing was successfully performed on 29 of 30 air-dried CTT, 27 of 30 ethanol-fixed CTT, and 27 of 30 FFPE tissues. The results exhibited 11, 13, and 13 BRAF mutations and 18, 14, and 14 wild types for the air-dried CTT, the ethanol-fixed CTT, and the FFPE tissues, respectively. The concordance rate was 96% between air-dried and ethanol-fixed CTT tissues, 88% between air-dried CTT and FFPE tissues, and 92% between ethanol-fixed CTT and FFPE tissues.CONCLUSIONS: PCR-based BRAF mutational testing can be reliably performed on the direct smears of the thyroid FNA specimens through the application of CTT.

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