Detection of filamentous tau inclusions by the fluorescent Congo red derivative FSB [(trans,trans)-1-fluoro-2,5-bis(3-hydroxycarbonyl-4-hydroxy)styrylbenzene]

Ana Velasco, Graham Fraser, Patrice Delobel, Bernardino Ghetti, Isabelle Lavenir, Michel Goedert

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Filamentous inclusions made of the microtubule-associated protein tau in a hyperphosphorylated state are a defining feature of a large number of human neurodegenerative diseases. Here we show that (trans,trans)-1-fluoro-2,5-bis(3-hydroxycarbonyl-4-hydroxy)styrylbenzene (FSB), a fluorescent Congo red derivative, labels tau inclusions in tissue sections from a mouse line transgenic for human P301S tau and in cases of familial frontotemporal dementia and sporadic Pick's disease. Labelling by FSB required the presence of tau filaments. More importantly, tau inclusions in the spinal cord of human P301S tau transgenic mice were labelled following a single intravenous injection of FSB. These findings indicate that FSB can be used to detect filamentous tau in vivo.

Original languageEnglish (US)
Pages (from-to)901-906
Number of pages6
JournalFEBS Letters
Volume582
Issue number6
DOIs
StatePublished - Mar 19 2008

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Keywords

  • Amyloid
  • Congo red
  • Filamentous inclusion
  • Live imaging
  • Tauopathies

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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