Detection of green turtle herpesviral sequence in saddleback wrasse Thalassoma duperrey

A possible mode of transmission of green turtle fibropapilloma.

Yuanan Lu, Qigui Yu, Andy Yu, Yun Wang, George S. Losey, George H. Balazs, Vivek R. Nerurkar, Richard Yanagihara

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Samples of DNA were prepared from various tissues and organs (including snout, gill, eye, brain, heart, liver, gut content, intestine, swim bladder, spleen, gallbladder, spinal cord, gonad, and muscle) of six healthy appearing reef cleaner fish, saddleback wrasses Thalassoma duperrey, captured from a cleaning station in North Kaneohe Bay, Oahu, Hawaii. The DNA samples were tested for evidence of green turtle herpesvirus infection by nested polymerase chain reaction (PCR). Green turtle herpesviral sequences were detected in snout (3/6), gill (2/6), and liver (1/6). All other tissues were negative. Except for a single nucleotide substitution (from A to G at position 48, resulting in a single amino acid change from isoleucine to methionine at position 16), the DNA sequences detected in the fish were identical to that of a newly reported green turtle herpesvirus. Although preliminary, these data represent the first evidence for an association of a herpesvirus with saddleback wrasse, suggesting that cleaner fish may serve as vectors or carriers for the transmission of the agent causing green turtle fibropapilloma.

Original languageEnglish (US)
Pages (from-to)58-63
Number of pages6
JournalJournal of Aquatic Animal Health
Volume12
Issue number1
StatePublished - Mar 2000
Externally publishedYes

Fingerprint

Thalassoma
Chelonia mydas
Turtles
Labridae
turtle
cleaner fish
Fishes
cleaners
Herpesviridae
DNA
gills
fish
swim bladder
Oahu
Herpesviridae Infections
liver
Isoleucine
gall bladder
Liver
Gonads

ASJC Scopus subject areas

  • Aquatic Science
  • veterinary(all)

Cite this

Detection of green turtle herpesviral sequence in saddleback wrasse Thalassoma duperrey : A possible mode of transmission of green turtle fibropapilloma. / Lu, Yuanan; Yu, Qigui; Yu, Andy; Wang, Yun; Losey, George S.; Balazs, George H.; Nerurkar, Vivek R.; Yanagihara, Richard.

In: Journal of Aquatic Animal Health, Vol. 12, No. 1, 03.2000, p. 58-63.

Research output: Contribution to journalArticle

Lu, Yuanan ; Yu, Qigui ; Yu, Andy ; Wang, Yun ; Losey, George S. ; Balazs, George H. ; Nerurkar, Vivek R. ; Yanagihara, Richard. / Detection of green turtle herpesviral sequence in saddleback wrasse Thalassoma duperrey : A possible mode of transmission of green turtle fibropapilloma. In: Journal of Aquatic Animal Health. 2000 ; Vol. 12, No. 1. pp. 58-63.
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