Detection of hepatitis C virus with RNA polymerase chain reaction in fulminant hepatic failure

Federico G. Villamil, Ke Qin Hu, Chang Hong Yu, Chao-Hung Lee, Sergio E. Rojter, Luis G. Podesta, Leonard Makowka, Stephen A. Geller, John M. Vierling

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The role of hepatitis C virus (HCV) infection in fulminant hepatic failure is controversial. The frequency of serum HCV RNA positivity in previously reported patients with fulminant hepatic failure (FHF) of indeterminate cause ranged from 0 to 12% in the United States and Europe and from 43% to 59% in Asia. We assessed serum HCV RNA using polymerase chain reaction (PCR) and oligoprimers from the 5′UTR of the HCV genome in 26 consecutive patients with FHF. Another laboratory independently performed PCR on 21 of the serum samples using different oligoprimers from the 5′UTR and NS3 region of the HCV genome. Serum HCV RNA was detected in two of seven (28%) patients with hepatitis B, 9 of 15 (60%) with an indeterminate cause, and in none with hepatitis A (n = 2) or drug-induced hepatotoxicity (n = 2). HCV RNA PCR results were concordant between both laboratories in 17 of 21 (81%) of samples. In patients with an indeterminate cause, HCV RNA positivity was significantly associated with the transmission risk factor of low socioeconomic status and Hispanic ethnicity. Eighteen patients underwent liver transplantation (LT) and 15 (83%) survived. Among patients with FHF of indeterminate cause, recurrent or acquired HCV infection after transplantation occurred in three of five (60%) and one of four (25%) patients, respectively. Three of four (75%) patients with hepatitis C virus infection post-LT also developed histologic hepatitis. HCV appears to be the causative agent of a substantial number of cases of FHF classified as indeterminate in the Los Angeles area. Differences in patient populations or risk factors may explain the discordant incidences of HCV infection in FHF observed among different programs.

Original languageEnglish
Pages (from-to)1379-1386
Number of pages8
JournalHepatology
Volume22
Issue number5
DOIs
StatePublished - 1995

Fingerprint

Acute Liver Failure
DNA-Directed RNA Polymerases
Hepacivirus
Polymerase Chain Reaction
Virus Diseases
RNA
Serum
Liver Transplantation
Genome
Hepatitis A
Los Angeles
Hepatitis B
Hispanic Americans
Social Class
Hepatitis
Transplantation

ASJC Scopus subject areas

  • Hepatology

Cite this

Villamil, F. G., Hu, K. Q., Yu, C. H., Lee, C-H., Rojter, S. E., Podesta, L. G., ... Vierling, J. M. (1995). Detection of hepatitis C virus with RNA polymerase chain reaction in fulminant hepatic failure. Hepatology, 22(5), 1379-1386. https://doi.org/10.1016/0270-9139(95)90140-X

Detection of hepatitis C virus with RNA polymerase chain reaction in fulminant hepatic failure. / Villamil, Federico G.; Hu, Ke Qin; Yu, Chang Hong; Lee, Chao-Hung; Rojter, Sergio E.; Podesta, Luis G.; Makowka, Leonard; Geller, Stephen A.; Vierling, John M.

In: Hepatology, Vol. 22, No. 5, 1995, p. 1379-1386.

Research output: Contribution to journalArticle

Villamil, FG, Hu, KQ, Yu, CH, Lee, C-H, Rojter, SE, Podesta, LG, Makowka, L, Geller, SA & Vierling, JM 1995, 'Detection of hepatitis C virus with RNA polymerase chain reaction in fulminant hepatic failure', Hepatology, vol. 22, no. 5, pp. 1379-1386. https://doi.org/10.1016/0270-9139(95)90140-X
Villamil, Federico G. ; Hu, Ke Qin ; Yu, Chang Hong ; Lee, Chao-Hung ; Rojter, Sergio E. ; Podesta, Luis G. ; Makowka, Leonard ; Geller, Stephen A. ; Vierling, John M. / Detection of hepatitis C virus with RNA polymerase chain reaction in fulminant hepatic failure. In: Hepatology. 1995 ; Vol. 22, No. 5. pp. 1379-1386.
@article{275b5ec5b5334ef087134e665c55f230,
title = "Detection of hepatitis C virus with RNA polymerase chain reaction in fulminant hepatic failure",
abstract = "The role of hepatitis C virus (HCV) infection in fulminant hepatic failure is controversial. The frequency of serum HCV RNA positivity in previously reported patients with fulminant hepatic failure (FHF) of indeterminate cause ranged from 0 to 12{\%} in the United States and Europe and from 43{\%} to 59{\%} in Asia. We assessed serum HCV RNA using polymerase chain reaction (PCR) and oligoprimers from the 5′UTR of the HCV genome in 26 consecutive patients with FHF. Another laboratory independently performed PCR on 21 of the serum samples using different oligoprimers from the 5′UTR and NS3 region of the HCV genome. Serum HCV RNA was detected in two of seven (28{\%}) patients with hepatitis B, 9 of 15 (60{\%}) with an indeterminate cause, and in none with hepatitis A (n = 2) or drug-induced hepatotoxicity (n = 2). HCV RNA PCR results were concordant between both laboratories in 17 of 21 (81{\%}) of samples. In patients with an indeterminate cause, HCV RNA positivity was significantly associated with the transmission risk factor of low socioeconomic status and Hispanic ethnicity. Eighteen patients underwent liver transplantation (LT) and 15 (83{\%}) survived. Among patients with FHF of indeterminate cause, recurrent or acquired HCV infection after transplantation occurred in three of five (60{\%}) and one of four (25{\%}) patients, respectively. Three of four (75{\%}) patients with hepatitis C virus infection post-LT also developed histologic hepatitis. HCV appears to be the causative agent of a substantial number of cases of FHF classified as indeterminate in the Los Angeles area. Differences in patient populations or risk factors may explain the discordant incidences of HCV infection in FHF observed among different programs.",
author = "Villamil, {Federico G.} and Hu, {Ke Qin} and Yu, {Chang Hong} and Chao-Hung Lee and Rojter, {Sergio E.} and Podesta, {Luis G.} and Leonard Makowka and Geller, {Stephen A.} and Vierling, {John M.}",
year = "1995",
doi = "10.1016/0270-9139(95)90140-X",
language = "English",
volume = "22",
pages = "1379--1386",
journal = "Hepatology",
issn = "0270-9139",
publisher = "John Wiley and Sons Ltd",
number = "5",

}

TY - JOUR

T1 - Detection of hepatitis C virus with RNA polymerase chain reaction in fulminant hepatic failure

AU - Villamil, Federico G.

AU - Hu, Ke Qin

AU - Yu, Chang Hong

AU - Lee, Chao-Hung

AU - Rojter, Sergio E.

AU - Podesta, Luis G.

AU - Makowka, Leonard

AU - Geller, Stephen A.

AU - Vierling, John M.

PY - 1995

Y1 - 1995

N2 - The role of hepatitis C virus (HCV) infection in fulminant hepatic failure is controversial. The frequency of serum HCV RNA positivity in previously reported patients with fulminant hepatic failure (FHF) of indeterminate cause ranged from 0 to 12% in the United States and Europe and from 43% to 59% in Asia. We assessed serum HCV RNA using polymerase chain reaction (PCR) and oligoprimers from the 5′UTR of the HCV genome in 26 consecutive patients with FHF. Another laboratory independently performed PCR on 21 of the serum samples using different oligoprimers from the 5′UTR and NS3 region of the HCV genome. Serum HCV RNA was detected in two of seven (28%) patients with hepatitis B, 9 of 15 (60%) with an indeterminate cause, and in none with hepatitis A (n = 2) or drug-induced hepatotoxicity (n = 2). HCV RNA PCR results were concordant between both laboratories in 17 of 21 (81%) of samples. In patients with an indeterminate cause, HCV RNA positivity was significantly associated with the transmission risk factor of low socioeconomic status and Hispanic ethnicity. Eighteen patients underwent liver transplantation (LT) and 15 (83%) survived. Among patients with FHF of indeterminate cause, recurrent or acquired HCV infection after transplantation occurred in three of five (60%) and one of four (25%) patients, respectively. Three of four (75%) patients with hepatitis C virus infection post-LT also developed histologic hepatitis. HCV appears to be the causative agent of a substantial number of cases of FHF classified as indeterminate in the Los Angeles area. Differences in patient populations or risk factors may explain the discordant incidences of HCV infection in FHF observed among different programs.

AB - The role of hepatitis C virus (HCV) infection in fulminant hepatic failure is controversial. The frequency of serum HCV RNA positivity in previously reported patients with fulminant hepatic failure (FHF) of indeterminate cause ranged from 0 to 12% in the United States and Europe and from 43% to 59% in Asia. We assessed serum HCV RNA using polymerase chain reaction (PCR) and oligoprimers from the 5′UTR of the HCV genome in 26 consecutive patients with FHF. Another laboratory independently performed PCR on 21 of the serum samples using different oligoprimers from the 5′UTR and NS3 region of the HCV genome. Serum HCV RNA was detected in two of seven (28%) patients with hepatitis B, 9 of 15 (60%) with an indeterminate cause, and in none with hepatitis A (n = 2) or drug-induced hepatotoxicity (n = 2). HCV RNA PCR results were concordant between both laboratories in 17 of 21 (81%) of samples. In patients with an indeterminate cause, HCV RNA positivity was significantly associated with the transmission risk factor of low socioeconomic status and Hispanic ethnicity. Eighteen patients underwent liver transplantation (LT) and 15 (83%) survived. Among patients with FHF of indeterminate cause, recurrent or acquired HCV infection after transplantation occurred in three of five (60%) and one of four (25%) patients, respectively. Three of four (75%) patients with hepatitis C virus infection post-LT also developed histologic hepatitis. HCV appears to be the causative agent of a substantial number of cases of FHF classified as indeterminate in the Los Angeles area. Differences in patient populations or risk factors may explain the discordant incidences of HCV infection in FHF observed among different programs.

UR - http://www.scopus.com/inward/record.url?scp=0028806245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028806245&partnerID=8YFLogxK

U2 - 10.1016/0270-9139(95)90140-X

DO - 10.1016/0270-9139(95)90140-X

M3 - Article

C2 - 7590651

AN - SCOPUS:0028806245

VL - 22

SP - 1379

EP - 1386

JO - Hepatology

JF - Hepatology

SN - 0270-9139

IS - 5

ER -