Detection of human papillomavirus L1 protein in condylomata acuminata from various anatomical sites

Kara Wools-Kaloustian, Janine T. Bryan, Barry Katz, Michael Rodriguez, Thomas Davis, Darron Brown

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background and Objectives: Though human papillomavirus (HPV) DNA can be detected in the majority of condylomata acuminata, the major capsid protein of HPV (LI protein) can be detected in only 10% to 50% of lesions in immunohistochemi-cal assays. Goal of Study: To evaluate the association between anatomic location of genital HPV infections and the ability to detect HPV L1 protein. Methods: Condylomata acuminata from 49 male and 51 female patients were evaluated for L1 protein by immunohisto-chemistry. Thirty of these lesions were vulvar; 20 were cervical; 1 was vaginal; 45 were penile; and 4 were perianal. A quantitative analysis of L1-positive nuclei in the lesions was performed. Results: L1 protein was detected in 20 (20%) of all lesions. L1 protein was detected in seven (35%) of the cervical lesions compared to nine (12%) of exophytic condylomata acuminata of males and females (P = 0.038). A statistically significant difference was noted between cervical and exophytic condylomata acuminata lesions (46.6 vs. 7.8) (P - 0.0006). No association between oral contraceptive use and L1 protein detection was found. Conclusions: The major capsid protein of HPV can be detected in condylomata acuminata of the uterine cervix more often, and in higher quantities, than in lesions of the vulva or penis. Further studies are needed to assess the effects of sex hormones and the anatomic location of the infection as they relate to transmission of HPV.

Original languageEnglish
Pages (from-to)103-106
Number of pages4
JournalSexually Transmitted Diseases
Volume21
Issue number2
StatePublished - 1994

Fingerprint

Condylomata Acuminata
Capsid Proteins
Proteins
Vulva
Papillomavirus Infections
Penis
Gonadal Steroid Hormones
Oral Contraceptives
Cervix Uteri
Human papillomavirus HPV L1 protein
DNA
Infection

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases
  • Microbiology (medical)
  • Public Health, Environmental and Occupational Health

Cite this

Detection of human papillomavirus L1 protein in condylomata acuminata from various anatomical sites. / Wools-Kaloustian, Kara; Bryan, Janine T.; Katz, Barry; Rodriguez, Michael; Davis, Thomas; Brown, Darron.

In: Sexually Transmitted Diseases, Vol. 21, No. 2, 1994, p. 103-106.

Research output: Contribution to journalArticle

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abstract = "Background and Objectives: Though human papillomavirus (HPV) DNA can be detected in the majority of condylomata acuminata, the major capsid protein of HPV (LI protein) can be detected in only 10{\%} to 50{\%} of lesions in immunohistochemi-cal assays. Goal of Study: To evaluate the association between anatomic location of genital HPV infections and the ability to detect HPV L1 protein. Methods: Condylomata acuminata from 49 male and 51 female patients were evaluated for L1 protein by immunohisto-chemistry. Thirty of these lesions were vulvar; 20 were cervical; 1 was vaginal; 45 were penile; and 4 were perianal. A quantitative analysis of L1-positive nuclei in the lesions was performed. Results: L1 protein was detected in 20 (20{\%}) of all lesions. L1 protein was detected in seven (35{\%}) of the cervical lesions compared to nine (12{\%}) of exophytic condylomata acuminata of males and females (P = 0.038). A statistically significant difference was noted between cervical and exophytic condylomata acuminata lesions (46.6 vs. 7.8) (P - 0.0006). No association between oral contraceptive use and L1 protein detection was found. Conclusions: The major capsid protein of HPV can be detected in condylomata acuminata of the uterine cervix more often, and in higher quantities, than in lesions of the vulva or penis. Further studies are needed to assess the effects of sex hormones and the anatomic location of the infection as they relate to transmission of HPV.",
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AU - Davis, Thomas

AU - Brown, Darron

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N2 - Background and Objectives: Though human papillomavirus (HPV) DNA can be detected in the majority of condylomata acuminata, the major capsid protein of HPV (LI protein) can be detected in only 10% to 50% of lesions in immunohistochemi-cal assays. Goal of Study: To evaluate the association between anatomic location of genital HPV infections and the ability to detect HPV L1 protein. Methods: Condylomata acuminata from 49 male and 51 female patients were evaluated for L1 protein by immunohisto-chemistry. Thirty of these lesions were vulvar; 20 were cervical; 1 was vaginal; 45 were penile; and 4 were perianal. A quantitative analysis of L1-positive nuclei in the lesions was performed. Results: L1 protein was detected in 20 (20%) of all lesions. L1 protein was detected in seven (35%) of the cervical lesions compared to nine (12%) of exophytic condylomata acuminata of males and females (P = 0.038). A statistically significant difference was noted between cervical and exophytic condylomata acuminata lesions (46.6 vs. 7.8) (P - 0.0006). No association between oral contraceptive use and L1 protein detection was found. Conclusions: The major capsid protein of HPV can be detected in condylomata acuminata of the uterine cervix more often, and in higher quantities, than in lesions of the vulva or penis. Further studies are needed to assess the effects of sex hormones and the anatomic location of the infection as they relate to transmission of HPV.

AB - Background and Objectives: Though human papillomavirus (HPV) DNA can be detected in the majority of condylomata acuminata, the major capsid protein of HPV (LI protein) can be detected in only 10% to 50% of lesions in immunohistochemi-cal assays. Goal of Study: To evaluate the association between anatomic location of genital HPV infections and the ability to detect HPV L1 protein. Methods: Condylomata acuminata from 49 male and 51 female patients were evaluated for L1 protein by immunohisto-chemistry. Thirty of these lesions were vulvar; 20 were cervical; 1 was vaginal; 45 were penile; and 4 were perianal. A quantitative analysis of L1-positive nuclei in the lesions was performed. Results: L1 protein was detected in 20 (20%) of all lesions. L1 protein was detected in seven (35%) of the cervical lesions compared to nine (12%) of exophytic condylomata acuminata of males and females (P = 0.038). A statistically significant difference was noted between cervical and exophytic condylomata acuminata lesions (46.6 vs. 7.8) (P - 0.0006). No association between oral contraceptive use and L1 protein detection was found. Conclusions: The major capsid protein of HPV can be detected in condylomata acuminata of the uterine cervix more often, and in higher quantities, than in lesions of the vulva or penis. Further studies are needed to assess the effects of sex hormones and the anatomic location of the infection as they relate to transmission of HPV.

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