Detection of increased choline compounds with proton nuclear magnetic resonance spectroscopy subsequent to malignant transformation of human prostatic epithelial cells

E. Ackerstaff, Beth Pflug, J. B. Nelson, Z. M. Bhujwalla

Research output: Contribution to journalArticle

243 Citations (Scopus)

Abstract

In this study, a panel of normal human prostate cells (HPCs) and tumor cells derived from metastases were studied by 1H NMR spectroscopy to determine whether the malignant transformation of HPCs results in the elevation of choline compounds. Although an elevated choline signal has been observed previously in clinical studies, the contribution of the different Cho compounds to this elevation, as well as their quantification, has not been established until now. Here we have shown that HPCs derived from metastases exhibit significantly higher phosphocholine as well as glycerophosphocholine levels compared with normal prostate epithelial and stromal cells. Thus the elevation of the choline peak observed clinically in prostate cancer is attributable to an alteration of phospholipid metabolism and not simply to increased cell density, doubling time, or other nonspecific effects. Androgen deprivation of the androgen receptor-positive cell lines resulted in a significant increase of choline compounds after chronic androgen deprivation of the LNCaP cell line and in a decrease of choline compounds after a more acute androgen deprivation of the LAPC-4 cell line. These data strongly support the use of proton magnetic resonance spectroscopic imaging to detect the presence of prostate cancer for diagnosis, to detect response subsequent to androgen ablation therapy, and to detect recurrence.

Original languageEnglish (US)
Pages (from-to)3599-3603
Number of pages5
JournalCancer Research
Volume61
Issue number9
StatePublished - May 1 2001
Externally publishedYes

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Choline
Magnetic Resonance Spectroscopy
Epithelial Cells
Androgens
Prostate
Cell Line
Prostatic Neoplasms
Neoplasm Metastasis
Phosphorylcholine
Androgen Receptors
Stromal Cells
Protons
Phospholipids
Cell Count
Magnetic Resonance Imaging
Proton Magnetic Resonance Spectroscopy
Recurrence
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Detection of increased choline compounds with proton nuclear magnetic resonance spectroscopy subsequent to malignant transformation of human prostatic epithelial cells. / Ackerstaff, E.; Pflug, Beth; Nelson, J. B.; Bhujwalla, Z. M.

In: Cancer Research, Vol. 61, No. 9, 01.05.2001, p. 3599-3603.

Research output: Contribution to journalArticle

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