Detection of lipid-induced structural changes of the Marburg virus matrix protein VP40 using hydrogen/deuterium exchange-mass spectrometry

Kaveesha J. Wijesinghe, Sarah Urata, Nisha Bhattarai, Edgar E. Kooijman, Bernard S. Gerstman, Prem P. Chapagain, Sheng Li, Robert Stahelin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Marburg virus (MARV) is a lipid-enveloped virus from the Filoviridae family containing a negative sense RNA genome. One of the sevenMARVgenes encodes the matrix protein VP40, which forms a matrix layer beneath the plasma membrane inner leaflet to facilitate budding from the host cell. MARV VP40 (mVP40) has been shown to be a dimeric peripheral protein with a broad and flat basic surface that can associate with anionic phospholipids such as phosphatidylserine. Although a number of mVP40 cationic residues have been shown to facilitate binding to membranes containing anionic lipids, much less is known on how mVP40 assembles to form the matrix layer following membrane binding. Here we have used hydrogen/deuterium exchange (HDX) mass spectrometry to determine the solvent accessibility of mVP40 residues in the absence and presence of phosphatidylserine and phosphatidylinositol 4,5-bisphosphate. HDX analysis demonstrates that two basic loops in the mVP40 C-Terminal domain make important contributions to anionic membrane binding and also reveals a potential oligomerization interface in the C-Terminal domain as well as a conserved oligomerization interface in the mVP40 N-Terminal domain. Lipid binding assays confirm the role of the two basic patches elucidated with HD/X measurements, whereas molecular dynamics simulations and membrane insertion measurements complement these studies to demonstrate that mVP40 does not appreciably insert into the hydrocarbon region of anionic membranes in contrast to the matrix protein from Ebola virus. Taken together, we propose a model by which association of the mVP40 dimer with the anionic plasma membrane facilitates assembly of mVP40 oligomers.

Original languageEnglish (US)
Pages (from-to)6108-6122
Number of pages15
JournalJournal of Biological Chemistry
Volume292
Issue number15
DOIs
StatePublished - Apr 14 2017

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Marburgvirus
Deuterium
Viruses
Mass spectrometry
Hydrogen
Mass Spectrometry
Membranes
Lipids
Oligomerization
Phosphatidylserines
Cell membranes
Proteins
Filoviridae
Cell Membrane
Ebolavirus
Die casting inserts
Molecular Dynamics Simulation
Hydrocarbons
Phosphatidylinositols
Oligomers

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Detection of lipid-induced structural changes of the Marburg virus matrix protein VP40 using hydrogen/deuterium exchange-mass spectrometry. / Wijesinghe, Kaveesha J.; Urata, Sarah; Bhattarai, Nisha; Kooijman, Edgar E.; Gerstman, Bernard S.; Chapagain, Prem P.; Li, Sheng; Stahelin, Robert.

In: Journal of Biological Chemistry, Vol. 292, No. 15, 14.04.2017, p. 6108-6122.

Research output: Contribution to journalArticle

Wijesinghe, Kaveesha J. ; Urata, Sarah ; Bhattarai, Nisha ; Kooijman, Edgar E. ; Gerstman, Bernard S. ; Chapagain, Prem P. ; Li, Sheng ; Stahelin, Robert. / Detection of lipid-induced structural changes of the Marburg virus matrix protein VP40 using hydrogen/deuterium exchange-mass spectrometry. In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 15. pp. 6108-6122.
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