Detection of loss of heterozygosity on chromosome 9q22.3 in microdissected sporadic basal cell carcinoma

Tingliang Shen, Won Sang Park, Roland Böninirmal Saini, Tina Pham, Alex E. Lash, Alexander O. Vortmeyer, Zhengping Zhuang

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Identification of loss of heterozygosity (LOH) at specific genetic loci in cancer cells suggests the presence of a tumor suppressor gene within the deleted region. A basal cell carcinoma (BCC) susceptibility gene, human homolog of drosophila patched (PTC), has been recently cloned and localized on chromosome 9q22.3. Mutation and deletion of this region has been reported in BCCs using frozen tumor tissue. The objective of this study was to test whether LOH of human PTC on chromosome 9q22 could be detected in archival sporadic BCCs. We studied 20 randomly selected sporadic BCCs by microdissection and polymerase chain reaction using paraffin-embedded, formalin-fixed material on glass slides. In all cases, analysis was performed with the polymorphic markers D9S53, D9S15, D9S287, and D9S303. The LOH frequencies were 30%, 42%, 56%, and 75% with D9S15, D9S287, D9S53, and D9S303, respectively. LOH at 9q22 was identified in 12 of 20 cases (60%) with at least one marker. Seven cases showed LOH with two markers, two cases with three markers, and one case showed LOH with all four markers. The results indicate that BCC LOH can be frequently identified in paraffin-embedded BCC after routine processing.

Original languageEnglish (US)
Pages (from-to)284-287
Number of pages4
JournalHuman pathology
Volume30
Issue number3
DOIs
StatePublished - Mar 1999
Externally publishedYes

Keywords

  • Basal cell carcinoma
  • Chromosome 9
  • Drosophila patched
  • Loss of heterozygosity

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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