Detection of Luxol-fast-blue positive cells in human promyelocytic leukemia cell line HL-60

L. Lu, Hal Broxmeyer, Louis Pelus, M. Andreeff, M. A. Moore

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Differentiation of HL-60 cells toward the eosinophilic series has not been reported previously. Eosinophil granule specific staining with Luxol-fast-blue was used to determine if HL-60 cells could differentiate into the eosinophilic lineage. The specificity of the Luxol-fast-blue stain for cells of the eosinophilic series was substantiated by comparison of the staining of cells from a patient with an eosinophilic syndrome by Wright-Giemsa and Luxol-fast-blue. Luxol-fast-blue positivity was most notable in cells found in colonies formed from HL-60 clonogenic cells in semisolid agar medium. Colony and cluster formation was spontaneous but in the presence of medium conditioned by either human placental cells or the human monocyte-like cell line, GCT, Luxol-fast-blue positive colonies and clusters were detected at a higher frequency.

Original languageEnglish (US)
Pages (from-to)887-892
Number of pages6
JournalExperimental Hematology
Volume9
Issue number9
StatePublished - 1981
Externally publishedYes

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Leukemia
HL-60 Cells
Cell Line
Staining and Labeling
Conditioned Culture Medium
Eosinophils
Agar
Monocytes
Coloring Agents
Luxol Fast Blue MBS

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Detection of Luxol-fast-blue positive cells in human promyelocytic leukemia cell line HL-60. / Lu, L.; Broxmeyer, Hal; Pelus, Louis; Andreeff, M.; Moore, M. A.

In: Experimental Hematology, Vol. 9, No. 9, 1981, p. 887-892.

Research output: Contribution to journalArticle

Lu, L. ; Broxmeyer, Hal ; Pelus, Louis ; Andreeff, M. ; Moore, M. A. / Detection of Luxol-fast-blue positive cells in human promyelocytic leukemia cell line HL-60. In: Experimental Hematology. 1981 ; Vol. 9, No. 9. pp. 887-892.
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