Detection of Luxol-fast-blue positive cells in human promyelocytic leukemia cell line HL-60

L. Lu, H. E. Broxmeyer, L. M. Pelus, M. Andreeff, M. A. Moore

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Differentiation of HL-60 cells toward the eosinophilic series has not been reported previously. Eosinophil granule specific staining with Luxol-fast-blue was used to determine if HL-60 cells could differentiate into the eosinophilic lineage. The specificity of the Luxol-fast-blue stain for cells of the eosinophilic series was substantiated by comparison of the staining of cells from a patient with an eosinophilic syndrome by Wright-Giemsa and Luxol-fast-blue. Luxol-fast-blue positivity was most notable in cells found in colonies formed from HL-60 clonogenic cells in semisolid agar medium. Colony and cluster formation was spontaneous but in the presence of medium conditioned by either human placental cells or the human monocyte-like cell line, GCT, Luxol-fast-blue positive colonies and clusters were detected at a higher frequency.

Original languageEnglish (US)
Pages (from-to)887-892
Number of pages6
JournalExperimental Hematology
Volume9
Issue number9
StatePublished - Dec 1 1981

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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