Detection of replication competent retrovirus and lentivirus.

Lakshmi Sastry, Kenneth Cornetta

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Retroviral vectors based on murine leukemia viruses (MuLV) have been used in clinical investigations for over a decade. Alternative retroviruses, most notably vectors based on HIV-1 and other lentiviruses, are now entering into clinical trials. Although vectors are designed to be replication defective, recombination events during vector production could lead to the generation of replication competent retroviruses (RCR) or replication competent lentiviruses (RCL). Careful screening of vector prior to human use must insure that patients are not inadvertently exposed to RCR or RCL. We describe methods capable of detecting low levels of virus contamination and discuss the current regulatory guidelines for screening gene therapy products intended for human use.

Original languageEnglish (US)
Pages (from-to)243-263
Number of pages21
JournalMethods in molecular biology (Clifton, N.J.)
Volume506
StatePublished - 2009

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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