Determination of clovoxamine concentration in human plasma by electron capture gas chromatography

H. E. Hurst, D. R. Jones, C. H. Jarboe, H. DeBree

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The antidepressant drug clovoxamine can be specifically and sensitively quantitated in human plasma by electron capture gas chromatography. Clovoxamine and the internal standard fluvoxamine are extracted into ethyl acetate from plasma at pH 12, back-extracted into phosphoric acid, and hydrolyzed at 90°C to form ketone derivatives, which are then re-extracted into hexane for injection into the chromatograph. As little as 1 μg of clovoxamine per liter of plasma can be measured. The coefficients of variation (CV) for the analysis at concentrations of 10 and 100 μg/L are respectively: within-run, 5.4% and 2.7%; between-run, 17.5% and 7.0%. When this assay was applied to plasma from individuals involved in an early clinical trial of clovoxamine, steady-state plasma concentrations ranged from 50 to 77 μg/L 3 h after a 50-mg oral dose of clovoxamine fumarate.

Original languageEnglish (US)
Pages (from-to)1210-1212
Number of pages3
JournalClinical chemistry
Volume27
Issue number7
DOIs
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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