Determination of clovoxamine concentration in human plasma by electron capture gas chromatography

H. E. Hurst, D. R. Jones, C. H. Jarboe, H. DeBree

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The antidepressant drug clovoxamine can be specifically and sensitively quantitated in human plasma by electron capture gas chromatography. Clovoxamine and the internal standard fluvoxamine are extracted into ethyl acetate from plasma at pH 12, back-extracted into phosphoric acid, and hydrolyzed at 90°C to form ketone derivatives, which are then re-extracted into hexane for injection into the chromatograph. As little as 1 μg of clovoxamine per liter of plasma can be measured. The coefficients of variation (CV) for the analysis at concentrations of 10 and 100 μg/L are respectively: within-run, 5.4% and 2.7%; between-run, 17.5% and 7.0%. When this assay was applied to plasma from individuals involved in an early clinical trial of clovoxamine, steady-state plasma concentrations ranged from 50 to 77 μg/L 3 h after a 50-mg oral dose of clovoxamine fumarate.

Original languageEnglish (US)
Pages (from-to)1210-1212
Number of pages3
JournalClinical Chemistry
Volume27
Issue number7
StatePublished - 1981
Externally publishedYes

Fingerprint

Plasma (human)
Gas chromatography
Gas Chromatography
Electrons
Plasmas
Fluvoxamine
Fumarates
Hexanes
Ketones
Antidepressive Agents
Assays
clovoxamine
Clinical Trials
Derivatives
Injections

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Hurst, H. E., Jones, D. R., Jarboe, C. H., & DeBree, H. (1981). Determination of clovoxamine concentration in human plasma by electron capture gas chromatography. Clinical Chemistry, 27(7), 1210-1212.

Determination of clovoxamine concentration in human plasma by electron capture gas chromatography. / Hurst, H. E.; Jones, D. R.; Jarboe, C. H.; DeBree, H.

In: Clinical Chemistry, Vol. 27, No. 7, 1981, p. 1210-1212.

Research output: Contribution to journalArticle

Hurst, HE, Jones, DR, Jarboe, CH & DeBree, H 1981, 'Determination of clovoxamine concentration in human plasma by electron capture gas chromatography', Clinical Chemistry, vol. 27, no. 7, pp. 1210-1212.
Hurst, H. E. ; Jones, D. R. ; Jarboe, C. H. ; DeBree, H. / Determination of clovoxamine concentration in human plasma by electron capture gas chromatography. In: Clinical Chemistry. 1981 ; Vol. 27, No. 7. pp. 1210-1212.
@article{99237975736e4dbca8e2a857e16cb816,
title = "Determination of clovoxamine concentration in human plasma by electron capture gas chromatography",
abstract = "The antidepressant drug clovoxamine can be specifically and sensitively quantitated in human plasma by electron capture gas chromatography. Clovoxamine and the internal standard fluvoxamine are extracted into ethyl acetate from plasma at pH 12, back-extracted into phosphoric acid, and hydrolyzed at 90°C to form ketone derivatives, which are then re-extracted into hexane for injection into the chromatograph. As little as 1 μg of clovoxamine per liter of plasma can be measured. The coefficients of variation (CV) for the analysis at concentrations of 10 and 100 μg/L are respectively: within-run, 5.4{\%} and 2.7{\%}; between-run, 17.5{\%} and 7.0{\%}. When this assay was applied to plasma from individuals involved in an early clinical trial of clovoxamine, steady-state plasma concentrations ranged from 50 to 77 μg/L 3 h after a 50-mg oral dose of clovoxamine fumarate.",
author = "Hurst, {H. E.} and Jones, {D. R.} and Jarboe, {C. H.} and H. DeBree",
year = "1981",
language = "English (US)",
volume = "27",
pages = "1210--1212",
journal = "Clinical Chemistry",
issn = "0009-9147",
publisher = "American Association for Clinical Chemistry Inc.",
number = "7",

}

TY - JOUR

T1 - Determination of clovoxamine concentration in human plasma by electron capture gas chromatography

AU - Hurst, H. E.

AU - Jones, D. R.

AU - Jarboe, C. H.

AU - DeBree, H.

PY - 1981

Y1 - 1981

N2 - The antidepressant drug clovoxamine can be specifically and sensitively quantitated in human plasma by electron capture gas chromatography. Clovoxamine and the internal standard fluvoxamine are extracted into ethyl acetate from plasma at pH 12, back-extracted into phosphoric acid, and hydrolyzed at 90°C to form ketone derivatives, which are then re-extracted into hexane for injection into the chromatograph. As little as 1 μg of clovoxamine per liter of plasma can be measured. The coefficients of variation (CV) for the analysis at concentrations of 10 and 100 μg/L are respectively: within-run, 5.4% and 2.7%; between-run, 17.5% and 7.0%. When this assay was applied to plasma from individuals involved in an early clinical trial of clovoxamine, steady-state plasma concentrations ranged from 50 to 77 μg/L 3 h after a 50-mg oral dose of clovoxamine fumarate.

AB - The antidepressant drug clovoxamine can be specifically and sensitively quantitated in human plasma by electron capture gas chromatography. Clovoxamine and the internal standard fluvoxamine are extracted into ethyl acetate from plasma at pH 12, back-extracted into phosphoric acid, and hydrolyzed at 90°C to form ketone derivatives, which are then re-extracted into hexane for injection into the chromatograph. As little as 1 μg of clovoxamine per liter of plasma can be measured. The coefficients of variation (CV) for the analysis at concentrations of 10 and 100 μg/L are respectively: within-run, 5.4% and 2.7%; between-run, 17.5% and 7.0%. When this assay was applied to plasma from individuals involved in an early clinical trial of clovoxamine, steady-state plasma concentrations ranged from 50 to 77 μg/L 3 h after a 50-mg oral dose of clovoxamine fumarate.

UR - http://www.scopus.com/inward/record.url?scp=0019479110&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019479110&partnerID=8YFLogxK

M3 - Article

C2 - 7237785

AN - SCOPUS:0019479110

VL - 27

SP - 1210

EP - 1212

JO - Clinical Chemistry

JF - Clinical Chemistry

SN - 0009-9147

IS - 7

ER -