Determination of mouse bladder inflammatory response to E. coli lipopolysaccharide

Travis Jerde, D. E. Bjorling, H. Steinberg, T. Warner, R. Saban

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Evaluation of the severity of histologic changes associated with cystitis is often subjective and inconsistent from one sample to the next. The objective of this study was to establish a consistent, reproducible method to quantify histologic changes in a mouse model of lipopolysaccharide (LPS)-induced cystitis. Either LPS (n = 8) or pyrogen-free saline (n = 8) was instilled intravesically into the bladders of female C57bk-6 J mice. Twenty-four hours later, mice in these groups as well as eight untreated controls were sacrificed and bladders were removed, fixed in formalin, and stained with hematoxylin and eosin (H and E). A bladder inflammatory index (BII) was described by reviewing tissues for edema, leukocyte infiltration, and hemorrhage. Cross-sections were evaluated by a single pathologist in a blinded manner based on the objective BII described. The BII method for objectively analyzing bladder inflammation was effective and reproducible. Bladders instilled with LPS had significantly increased inflammation scores for edema, leukocyte infiltration, and hemorrhage compared with those instilled with saline or untreated controls (n = 8, P <0.05). These results demonstrate that LPS causes bladder inflammation when instilled intravesically and that inflammation of mouse bladders can be objectively quantified using the histological method described.

Original languageEnglish (US)
Pages (from-to)269-273
Number of pages5
JournalUrological Research
Volume28
Issue number4
StatePublished - 2000
Externally publishedYes

Fingerprint

Lipopolysaccharides
Urinary Bladder
Escherichia coli
Inflammation
Cystitis
Edema
Leukocytes
Hemorrhage
Pyrogens
Hematoxylin
Eosine Yellowish-(YS)
Formaldehyde

Keywords

  • Bladder
  • Histology
  • Inflammation
  • Lipopolysaccharide

ASJC Scopus subject areas

  • Urology

Cite this

Jerde, T., Bjorling, D. E., Steinberg, H., Warner, T., & Saban, R. (2000). Determination of mouse bladder inflammatory response to E. coli lipopolysaccharide. Urological Research, 28(4), 269-273.

Determination of mouse bladder inflammatory response to E. coli lipopolysaccharide. / Jerde, Travis; Bjorling, D. E.; Steinberg, H.; Warner, T.; Saban, R.

In: Urological Research, Vol. 28, No. 4, 2000, p. 269-273.

Research output: Contribution to journalArticle

Jerde, T, Bjorling, DE, Steinberg, H, Warner, T & Saban, R 2000, 'Determination of mouse bladder inflammatory response to E. coli lipopolysaccharide', Urological Research, vol. 28, no. 4, pp. 269-273.
Jerde, Travis ; Bjorling, D. E. ; Steinberg, H. ; Warner, T. ; Saban, R. / Determination of mouse bladder inflammatory response to E. coli lipopolysaccharide. In: Urological Research. 2000 ; Vol. 28, No. 4. pp. 269-273.
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