Determination of salsolinol and related catecholamines through on-line preconcentration and liquid chromatography/atmospheric pressure photoionization mass spectrometry

Jason A. Starkey, Yehia Mechref, Jan Muzikar, William J. McBride, Milos V. Novotny

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A new analytical approach has been developed for simultaneous measurements of endogenous salsolinol and major catecholamines in brain tissue of experimental animals. This procedure involves a combination of on-line phenyl boronate affinity preconcentration and microcolumn liquid chromatography, followed by mass spectrometry equipped with an atmospheric pressure photoionization (APPI) source. Flow conditions of the APPI source were optimized for detection sensitivity while different dopants were evaluated. The on-line preconcentration was found essential for the sensitivity requirements of salsolinol measurements in the brain tissue from alcohol-preferring rats subjected to different levels of alcohol exposure.

Original languageEnglish
Pages (from-to)3342-3347
Number of pages6
JournalAnalytical Chemistry
Volume78
Issue number10
DOIs
StatePublished - May 15 2006

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Photoionization
Liquid chromatography
Atmospheric pressure
Catecholamines
Mass spectrometry
Brain
Alcohols
Tissue
Rats
Animals
Doping (additives)
salsolinol

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Determination of salsolinol and related catecholamines through on-line preconcentration and liquid chromatography/atmospheric pressure photoionization mass spectrometry. / Starkey, Jason A.; Mechref, Yehia; Muzikar, Jan; McBride, William J.; Novotny, Milos V.

In: Analytical Chemistry, Vol. 78, No. 10, 15.05.2006, p. 3342-3347.

Research output: Contribution to journalArticle

Starkey, Jason A. ; Mechref, Yehia ; Muzikar, Jan ; McBride, William J. ; Novotny, Milos V. / Determination of salsolinol and related catecholamines through on-line preconcentration and liquid chromatography/atmospheric pressure photoionization mass spectrometry. In: Analytical Chemistry. 2006 ; Vol. 78, No. 10. pp. 3342-3347.
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