Determination of the promoter strength of the gene encoding Escherichia coli heat-stable enterotoxin II

Dan Spandau, Chao-Hung Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We studied the promoter strength of the gene encoding the Escherichia coli heat-stable enterotoxin II (STII). The promoter region and a portion of the 5' coding sequence of the STII gene were fused to the lacZ gene so that the production of β-galactosidase was under the control of the STII gene promoter. The strength of the STII gene promoter was compared with that of the ompF and lac operons, which were similarly fused to the lacZ gene. The β-galactosidase produced by the hybrid genes was assayed in vitro by using cell extracts. The mRNA transcribed by each promoter was assayed by Northern blot analysis and by in vitro transcription. The results suggest that the STII gene is regulated by a relatively weak promoter.

Original languageEnglish
Pages (from-to)1740-1744
Number of pages5
JournalJournal of Bacteriology
Volume169
Issue number4
StatePublished - 1987

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Enterotoxins
Escherichia coli
Lac Operon
Galactosidases
Genes
Cell Extracts
Genetic Promoter Regions
Northern Blotting
heat stable toxin (E coli)
Messenger RNA
In Vitro Techniques

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Determination of the promoter strength of the gene encoding Escherichia coli heat-stable enterotoxin II. / Spandau, Dan; Lee, Chao-Hung.

In: Journal of Bacteriology, Vol. 169, No. 4, 1987, p. 1740-1744.

Research output: Contribution to journalArticle

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